BMRB Entry 10134
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR10134
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Title: 1H and 15N assignment of oxidized Hydrogenobacter thermophilus cytochrome c552 PubMed: 17658890
Deposition date: 2007-04-15 Original release date: 2008-02-11
Authors: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki
Citation: Takayama, Shin-ichi; Takahashi, Yo-ta; Mikami, Shin-ichi; Irie, Kiyofumi; Kawano, Shin; Yamamoto, Yasuhiko; Hemmi, Hikaru; Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "Local conformational transition of Hydrogenobacter thermophilus cytochrome c552 relevant to its redox potential." Biochemistry 46, 9215-9224 (2007).
Assembly members:
cytochrome c552, polymer, 80 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.
Natural source: Common Name: Hydrogenobacter thermophilus Taxonomy ID: 940 Superkingdom: Eubacteria Kingdom: not available Genus/species: Hydrogenobacter thermophilus
Experimental source: Production method: recombinant technology Vector: pKK223-3
Entity Sequences (FASTA):
cytochrome c552: NEQLAKQKGCMACHDLKAKK
VGPAYADVAKKYAGRKDAVD
YLAGKIKKGGSGVWGSVPMP
PQNVTDAEAKQLAQWILSIK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 73 |
| 1H chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cytochrome c | 1 |
| 2 | oxidized heme | 2 |
Entities:
Entity 1, cytochrome c 80 residues - Formula weight is not available
| 1 | ASN | GLU | GLN | LEU | ALA | LYS | GLN | LYS | GLY | CYS | |
| 2 | MET | ALA | CYS | HIS | ASP | LEU | LYS | ALA | LYS | LYS | |
| 3 | VAL | GLY | PRO | ALA | TYR | ALA | ASP | VAL | ALA | LYS | |
| 4 | LYS | TYR | ALA | GLY | ARG | LYS | ASP | ALA | VAL | ASP | |
| 5 | TYR | LEU | ALA | GLY | LYS | ILE | LYS | LYS | GLY | GLY | |
| 6 | SER | GLY | VAL | TRP | GLY | SER | VAL | PRO | MET | PRO | |
| 7 | PRO | GLN | ASN | VAL | THR | ASP | ALA | GLU | ALA | LYS | |
| 8 | GLN | LEU | ALA | GLN | TRP | ILE | LEU | SER | ILE | LYS |
Entity 2, oxidized heme - C34 H34 Fe N4 O4 - 618.503 Da.
| 1 | HEC |
Samples:
sample_1: cytochrome c552, [U-15N], 1 mM; potassium phosphate 20 mM; potassium ferricyanide 10 mM
Cond_1: pH: 6.0; temperature: 296 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N HSQC | sample_1 | not available | Cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 10135 17053 25389 25390 |
| PDB | |
| DBJ | BAI69446 |
| EMBL | CAA40902 |
| GB | ADO45379 |
| REF | WP_012963626 |
| SP | P15452 |
| AlphaFold | P15452 |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
or all simulated shifts