BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11056

Title: KMTM7 a peptide derived from V-ATPase subunit a, putative TM7   PubMed: 19249284

Deposition date: 2008-11-07 Original release date: 2009-04-17

Authors: Vermeer, Louic; Reat, Valerie; Hemminga, Marcus; Milon, Alain

Citation: Vermeer, Louic; Reat, Valerie; Hemminga, Marcus; Milon, Alain. "Structural properties of a peptide derived from H(+)-V-ATPase subunit a"  Biochim. Biophys. Acta. 1788, 1204-1212 (2009).

Assembly members:
KMTM7, polymer, 25 residues, 2783 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
KMTM7: KKSHTASYLRLWALSLAHAQ LSSKK

Data sets:
Data typeCount
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KMTM71

Entities:

Entity 1, KMTM7 25 residues - 2783 Da.

the 2 lysine residues on both the N-term and C-term are non-native

1   LYSLYSSERHISTHRALASERTYRLEUARG
2   LEUTRPALALEUSERLEUALAHISALAGLN
3   LEUSERSERLYSLYS

Samples:

sample_1: KMTM7 1 mM; SDS, [U-100% 2H], 250 mM; sodium phosphate 10 mM; DSS 0.3 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

ARIA v2.2, Linge, O'Donoghue and Nilges - structure solution

AQUA v3.2, Rullmann, Doreleijers and Kaptein - data analysis

SPARKY, Goddard - chemical shift assignment, peak picking

ProcheckNMR, Laskowski and MacArthur - data analysis

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 15025
PDB
DBJ BAB62291 BAB62292 BAB62293 GAA25496 GAA26581
EMBL CAA20334 CAA61776 CAA77448 CAA77453 CAA89764
GB AAA35211 AAD34771 AAF28475 AAF53116 AAF55550
REF NP_001023017 NP_001023018 NP_001023019 NP_001023020 NP_001023021
SP P30628 P32563 P37296
TPG DAA09953 DAA11036
AlphaFold P30628 P37296 P32563