BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 1127

Title: Static and Transient Hydrogen-Bonding Interactions in Recombinant Desulfatohirudin Studied by 1H Nuclear Magnetic Resonance Measurements of Amide Proton Exchange Rates and pH-Dependent Chemical Shifts

Deposition date: 1995-07-31 Original release date: 1995-07-31

Authors: Haruyama, Hideyuki; Qian, Yan-qiu; Wuthrich, Kurt

Citation: Haruyama, Hideyuki; Qian, Yan-qiu; Wuthrich, Kurt. "Static and Transient Hydrogen-Bonding Interactions in Recombinant Desulfatohirudin Studied by 1H Nuclear Magnetic Resonance Measurements of Amide Proton Exchange Rates and pH-Dependent Chemical Shifts"  Biochemistry 28, 4312-4317 (1989).

Assembly members:
desulfatohirudin, polymer, 65 residues, Formula weight is not available

Natural source:   Common Name: leech   Taxonomy ID: 6421   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Hirudo medicinalis

Experimental source:   Production method: not available

Entity Sequences (FASTA):
desulfatohirudin: VVYTDCTESGQNLCLCEGSN VCGQGNKCILGSDGEKNQCV TGEGTPKPQSHNDGDFEEIP EEYLQ

Data sets:
Data typeCount
1H chemical shifts23

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1desulfatohirudin1

Entities:

Entity 1, desulfatohirudin 65 residues - Formula weight is not available

1   VALVALTYRTHRASPCYSTHRGLUSERGLY
2   GLNASNLEUCYSLEUCYSGLUGLYSERASN
3   VALCYSGLYGLNGLYASNLYSCYSILELEU
4   GLYSERASPGLYGLULYSASNGLNCYSVAL
5   THRGLYGLUGLYTHRPROLYSPROGLNSER
6   HISASNASPGLYASPPHEGLUGLUILEPRO
7   GLUGLUTYRLEUGLN

Samples:

sample_one:

sample_condition_set_one: pH: 4.5 na; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_condition_set_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

BMRB 1128 1129 27
PDB
GB AAA72556 AAA72772 AAA73216 AAF85971 AAG33034
PRF 1002207A
SP P01050 P28509 P28511
AlphaFold P28511 P28509 P01050