BMRB Entry 11400
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR11400
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Title: Solution Structure of the RNA recognition motif in Arginine/serine-rich splicing factor 10
Deposition date: 2010-09-09 Original release date: 2011-09-08
Authors: Someya, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.
Citation: Someya, T.; Muto, Y.; Inoue, M.; Kigawa, T.; Terada, T.; Shirouzu, M.; Yokoyama, S.. "Solution Structure of the RNA recognition motif in Arginine/serine-rich splicing factor 10" .
Assembly members:
RNA recognition motif, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: P040517-05
Entity Sequences (FASTA):
RNA recognition motif: GSSGSSGNRANPDPNCCLGV
FGLSLYTTERDLREVFSKYG
PIADVSIVYDQQSRRSRGFA
FVYFENVDDAKEAKERANGM
ELDGRRIRVSGPSSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 365 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 564 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA recognition motif | 1 |
Entities:
Entity 1, RNA recognition motif 95 residues - Formula weight is not available
| 1 | GLY | SER | SER | GLY | SER | SER | GLY | ASN | ARG | ALA | ||||
| 2 | ASN | PRO | ASP | PRO | ASN | CYS | CYS | LEU | GLY | VAL | ||||
| 3 | PHE | GLY | LEU | SER | LEU | TYR | THR | THR | GLU | ARG | ||||
| 4 | ASP | LEU | ARG | GLU | VAL | PHE | SER | LYS | TYR | GLY | ||||
| 5 | PRO | ILE | ALA | ASP | VAL | SER | ILE | VAL | TYR | ASP | ||||
| 6 | GLN | GLN | SER | ARG | ARG | SER | ARG | GLY | PHE | ALA | ||||
| 7 | PHE | VAL | TYR | PHE | GLU | ASN | VAL | ASP | ASP | ALA | ||||
| 8 | LYS | GLU | ALA | LYS | GLU | ARG | ALA | ASN | GLY | MET | ||||
| 9 | GLU | LEU | ASP | GLY | ARG | ARG | ILE | ARG | VAL | SER | ||||
| 10 | GLY | PRO | SER | SER | GLY |
Samples:
sample_1: RNA recognition motif, [U-13C; U-15N], 1.14 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%
condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | sample_1 | isotropic | condition_1 |
| 3D 13C-separated NOESY | sample_1 | isotropic | condition_1 |
Software:
xwinnmr v3.5, Bruker - collection
NMRPipe v20030801, Delaglio, F. - processing
NMRView v5.0.4, Johnson, B.A. - data analysis
Kujira v0.9295, Kobayashi, N. - data analysis
CYANA v2.0.17, Guntert, P. - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 800 MHz
Related Database Links:
| BMRB | 11409 11410 11411 11412 16920 |
| PDB | |
| DBJ | BAA08556 BAC05256 BAC33819 BAD92445 BAE88784 |
| EMBL | CAA56518 CAH18071 CAH90742 |
| GB | AAB08701 AAC28242 AAD19277 AAD19278 AAG35783 |
| REF | NP_001006878 NP_001029948 NP_001070689 NP_001125414 NP_001230808 |
| SP | P62995 P62996 P62997 Q3ZBT6 |
| TPG | DAA33375 |
| AlphaFold | P62996 P62997 P62995 Q3ZBT6 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts