BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11484

Title: Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT

Deposition date: 2012-03-06 Original release date: 2015-01-20

Authors: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki

Citation: Tochio, Naoya; Umehara, Takashi; Kigawa, Takanori; Yokoyama, Shigeyuki. "Solution structures of the DNA-binding domains of immune-related zinc-finger protein ZFAT"  .

Assembly members:
protein, polymer, 37 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P060718-11

Entity Sequences (FASTA):
protein: GSSGSSGIKQHCRFCKKKYS DVKNLIKHIRDMHDPQD

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts31
1H chemical shifts230

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2ZINC ION2

Entities:

Entity 1, protein 37 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYILELYSGLN
2   HISCYSARGPHECYSLYSLYSLYSTYRSER
3   ASPVALLYSASNLEUILELYSHISILEARG
4   ASPMETHISASPPROGLNASP

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: protein, [U-13C; U-15N], 1.09 mM; TRIS, [U-2H], 20 mM; sodium chloride 100 mM; DTT, [U-2H], 1 mM; sodium azide 0.02%; zinc chloride 50 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 296 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

xwinnmr, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 11476
PDB
GB AAH53930 ABY67968 EDL29394 EDL29395 EDM16136
REF NP_001128429 NP_001139360 NP_941046 XP_005078763 XP_006241774
SP Q7TS63
AlphaFold Q7TS63

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts