BMRB Entry 15070
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15070
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR Assignments of the apo Corynebacterium diphtheria Heme Oxygenase-G135A PubMed: 19636825
Deposition date: 2006-12-07 Original release date: 2007-06-26
Authors: Hom, Kellie
Citation: Hom, Kellie; Furci, Lena; Deshmukh, Rahul; Wilks, Angela. "NMR assignments of cd-HO, a 24 kDa heme oxygenase from Corynebacterium diphtheria" Biomol. NMR Assignments 1, 55-56 (2007).
Assembly members:
heme_oxygenase, polymer, 215 residues, 24152 Da.
Natural source: Common Name: Corynebacterium diphtheria Taxonomy ID: 1717 Superkingdom: Bacteria Kingdom: not available Genus/species: Corynebacterium diphtheriae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21a
Entity Sequences (FASTA):
heme_oxygenase: MTTATAGLAVELKQSTAQAH
EKAEHSTFMSDLLKGRLGVA
EFTRLQEQAWLFYTALEQAV
DAVRASGFAESLLDPALNRA
EVLARDLDKLNGSSEWRSRI
TASPAVIDYVNRLEEIRDNV
DGPALVAHHYVRYLADLSGG
QVIARMMQRHYGVDPEALGF
YHFEGIAKLKVYKDEYREKL
NNLELSDEQREHLLKEATDA
FVFNHQVFADLGKGL
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 571 |
15N chemical shifts | 190 |
1H chemical shifts | 191 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | heme oxygenase | 1 |
Entities:
Entity 1, heme oxygenase 215 residues - 24152 Da.
1 | MET | THR | THR | ALA | THR | ALA | GLY | LEU | ALA | VAL | ||||
2 | GLU | LEU | LYS | GLN | SER | THR | ALA | GLN | ALA | HIS | ||||
3 | GLU | LYS | ALA | GLU | HIS | SER | THR | PHE | MET | SER | ||||
4 | ASP | LEU | LEU | LYS | GLY | ARG | LEU | GLY | VAL | ALA | ||||
5 | GLU | PHE | THR | ARG | LEU | GLN | GLU | GLN | ALA | TRP | ||||
6 | LEU | PHE | TYR | THR | ALA | LEU | GLU | GLN | ALA | VAL | ||||
7 | ASP | ALA | VAL | ARG | ALA | SER | GLY | PHE | ALA | GLU | ||||
8 | SER | LEU | LEU | ASP | PRO | ALA | LEU | ASN | ARG | ALA | ||||
9 | GLU | VAL | LEU | ALA | ARG | ASP | LEU | ASP | LYS | LEU | ||||
10 | ASN | GLY | SER | SER | GLU | TRP | ARG | SER | ARG | ILE | ||||
11 | THR | ALA | SER | PRO | ALA | VAL | ILE | ASP | TYR | VAL | ||||
12 | ASN | ARG | LEU | GLU | GLU | ILE | ARG | ASP | ASN | VAL | ||||
13 | ASP | GLY | PRO | ALA | LEU | VAL | ALA | HIS | HIS | TYR | ||||
14 | VAL | ARG | TYR | LEU | ALA | ASP | LEU | SER | GLY | GLY | ||||
15 | GLN | VAL | ILE | ALA | ARG | MET | MET | GLN | ARG | HIS | ||||
16 | TYR | GLY | VAL | ASP | PRO | GLU | ALA | LEU | GLY | PHE | ||||
17 | TYR | HIS | PHE | GLU | GLY | ILE | ALA | LYS | LEU | LYS | ||||
18 | VAL | TYR | LYS | ASP | GLU | TYR | ARG | GLU | LYS | LEU | ||||
19 | ASN | ASN | LEU | GLU | LEU | SER | ASP | GLU | GLN | ARG | ||||
20 | GLU | HIS | LEU | LEU | LYS | GLU | ALA | THR | ASP | ALA | ||||
21 | PHE | VAL | PHE | ASN | HIS | GLN | VAL | PHE | ALA | ASP | ||||
22 | LEU | GLY | LYS | GLY | LEU |
Samples:
sample_1: heme oxygenase, [U-13C; U-15N; U-2H], 1 2 mM; H2O 95%; D2O 5%; potassium phosphate 50 mM; potassium chloride 100 mM; DSS 0.5 mM
sample_conditions_1: ionic strength: 100 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY v3.11, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 500 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts