BMRB Entry 15445
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15445
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Title: 15N relaxation and H/D exchange analysis of 15.5K free in solution PubMed: 18044964
Deposition date: 2007-08-27 Original release date: 2008-06-26
Authors: Soss, Sarah; Flynn, Peter
Citation: Soss, Sarah; Flynn, Peter. "Functional implications for a prototypical K-turn binding protein from structural and dynamical studies of 15.5K" Biochemistry 46, 14979-14986 (2007).
Assembly members:
15.5K, polymer, 144 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
15.5K: MGHHHHHHSSGIEEGRMTEA
DVNPKAYPLADAHLTKKLLD
LVQQSCNYKQLRKGANEATK
TLNRGISEFIVMAADAEPLE
IILHLPLLCEDKNVPYVFVR
SKQALGRACGVSRPVIACSV
TIKEGSQLKQQIQSIQQSIE
RLLV
- heteronucl_NOEs
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- H_exch_protection_factors
- H_exch_rates
- order_parameters
| Data type | Count |
| heteronuclear NOE values | 186 |
| H exchange protection factors | 61 |
| H exchange rates | 61 |
| order parameters | 95 |
| T1 relaxation values | 191 |
| T2 relaxation values | 189 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 15.5K | 1 |
Entities:
Entity 1, 15.5K 144 residues - Formula weight is not available
The first 16 residues are an affinity tag, numbering begins with M1.
| 1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | ||||
| 2 | GLY | ILE | GLU | GLU | GLY | ARG | MET | THR | GLU | ALA | ||||
| 3 | ASP | VAL | ASN | PRO | LYS | ALA | TYR | PRO | LEU | ALA | ||||
| 4 | ASP | ALA | HIS | LEU | THR | LYS | LYS | LEU | LEU | ASP | ||||
| 5 | LEU | VAL | GLN | GLN | SER | CYS | ASN | TYR | LYS | GLN | ||||
| 6 | LEU | ARG | LYS | GLY | ALA | ASN | GLU | ALA | THR | LYS | ||||
| 7 | THR | LEU | ASN | ARG | GLY | ILE | SER | GLU | PHE | ILE | ||||
| 8 | VAL | MET | ALA | ALA | ASP | ALA | GLU | PRO | LEU | GLU | ||||
| 9 | ILE | ILE | LEU | HIS | LEU | PRO | LEU | LEU | CYS | GLU | ||||
| 10 | ASP | LYS | ASN | VAL | PRO | TYR | VAL | PHE | VAL | ARG | ||||
| 11 | SER | LYS | GLN | ALA | LEU | GLY | ARG | ALA | CYS | GLY | ||||
| 12 | VAL | SER | ARG | PRO | VAL | ILE | ALA | CYS | SER | VAL | ||||
| 13 | THR | ILE | LYS | GLU | GLY | SER | GLN | LEU | LYS | GLN | ||||
| 14 | GLN | ILE | GLN | SER | ILE | GLN | GLN | SER | ILE | GLU | ||||
| 15 | ARG | LEU | LEU | VAL |
Samples:
sample_1: 15.5K, [U-99% 15N], 0.8 mM; D2O 8%; sodium azide 0.1%; sodium chloride 100 mM; sodium phosphate 100 mM
sample_2: 15.5K, [U-99% 15N], 0.6 mM; D2O 95%; H2O 5%; sodium chloride 100 mM; sodium phosphate 100 mM
sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K
sample_conditions_2: pD: 6.52; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_2 | isotropic | sample_conditions_2 |
Software:
FELIX v95.0, Accelrys Software Inc. - peak picking, processing
relxn2.2, Dr. Andrew Lee - processing
NMR spectrometers:
- Varian INOVA 500 MHz
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAA23363 BAB23329 BAE01699 BAE22349 BAE26537 |
| EMBL | CAG30417 CAG46526 CAK54562 CAK54861 |
| GB | AAC72945 AAF06959 AAH05358 AAH19282 AAH26755 |
| PRF | 2210268A |
| REF | NP_001003796 NP_001070472 NP_001177155 NP_001177156 NP_001271884 |
| SP | P55769 P55770 Q3B8S0 Q4R5C6 Q9D0T1 |
| TPG | DAA29093 |
| AlphaFold | Q9D0T1 P55769 P55770 Q3B8S0 Q4R5C6 |