BMRB Entry 16275
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16275
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Title: Apelin17 bound to DPC micelles PubMed: 19708686
Deposition date: 2009-05-05 Original release date: 2009-08-04
Authors: Langelaan, David; Rainey, Jan
Citation: Langelaan, David; Rainey, Jan. "Headgroup-Dependent Membrane Catalysis of Apelin-Receptor Interactions Is Likely" J. Phys. Chem. B. 113, 10465-10471 (2009).
Assembly members:
Apelin-17, polymer, 17 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Apelin-17: KFRRQRPRLSHKGPMPF
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
13C chemical shifts | 69 |
15N chemical shifts | 13 |
1H chemical shifts | 134 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Apelin-17 | 1 |
Entities:
Entity 1, Apelin-17 17 residues - Formula weight is not available
1 | LYS | PHE | ARG | ARG | GLN | ARG | PRO | ARG | LEU | SER | ||||
2 | HIS | LYS | GLY | PRO | MET | PRO | PHE |
Samples:
sample_1: Apelin-17 0.5 mM; D2O 10%; sodium acetate 20 mM; Sodium Azide 1 mM; DSS 1 mM; Dodecyl phosphocholine 94 mM
sample_conditions_1: ionic strength: 21 mM; pH: 5; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Varian INOVA 800 MHz
- Bruker Avance 500 MHz
- Bruker Avance 700 MHz
Related Database Links:
BMRB | 20029 20030 20031 20082 |
DBJ | BAA84974 BAA84975 BAA84976 BAA84977 BAC31156 |
EMBL | CAC83678 |
GB | AAF25814 AAF25815 AAH20015 AAH21104 AAH80843 |
REF | NP_001177797 NP_038940 NP_059109 NP_113800 NP_776928 |
SP | Q9R0R3 Q9R0R4 Q9TUI9 Q9ULZ1 |
TPG | DAA13389 |
AlphaFold | Q9R0R4 Q9R0R3 Q9TUI9 Q9ULZ1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts