BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 16726

Title: Resonance assignment of myristoylated Neuronal Calcium Sensor 1(NCS1) in membrane bound form.   PubMed: 20490953

Deposition date: 2010-02-15 Original release date: 2011-02-15

Authors: Chary, Kandala; Chandra, Kousik; Ramakrishnan, Venkatesh; Sharma, Yogendra

Citation: Chandra, Kousik; Ramakrishnan, Venkatesh; Sharma, Yogendra; Chary, Kandala. "Resonance assignments of myristoylated and non-myristoylated neuronal calcium sensor-1(NCS-1) embedded in a membrane"  Biomol. NMR Assignments 4, 155-158 (2010).

Assembly members:
myristoylated NCS1, polymer, 190 residues, Formula weight is not available

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):
myristoylated NCS1: MGKSNSKLKPEVVEELTRKT YFTEKEVQQWYKGFIKDCPS GQLDAAGFQKIYKQFFPFGD PTKFATFVFNVFDENKDGRI EFSEFIQALSVTSRGTLDEK LRWAFKLYDLDNDGYITRNE MLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNA DGKLTLQEFQEGSKADPSIV QALSLYDGLV

Data sets:
Data typeCount
15N chemical shifts176
1H chemical shifts1072

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1myristoylated NCS11

Entities:

Entity 1, myristoylated NCS1 190 residues - Formula weight is not available

1   METGLYLYSSERASNSERLYSLEULYSPRO
2   GLUVALVALGLUGLULEUTHRARGLYSTHR
3   TYRPHETHRGLULYSGLUVALGLNGLNTRP
4   TYRLYSGLYPHEILELYSASPCYSPROSER
5   GLYGLNLEUASPALAALAGLYPHEGLNLYS
6   ILETYRLYSGLNPHEPHEPROPHEGLYASP
7   PROTHRLYSPHEALATHRPHEVALPHEASN
8   VALPHEASPGLUASNLYSASPGLYARGILE
9   GLUPHESERGLUPHEILEGLNALALEUSER
10   VALTHRSERARGGLYTHRLEUASPGLULYS
11   LEUARGTRPALAPHELYSLEUTYRASPLEU
12   ASPASNASPGLYTYRILETHRARGASNGLU
13   METLEUASPILEVALASPALAILETYRGLN
14   METVALGLYASNTHRVALGLULEUPROGLU
15   GLUGLUASNTHRPROGLULYSARGVALASP
16   ARGILEPHEALAMETMETASPLYSASNALA
17   ASPGLYLYSLEUTHRLEUGLNGLUPHEGLN
18   GLUGLYSERLYSALAASPPROSERILEVAL
19   GLNALALEUSERLEUTYRASPGLYLEUVAL

Samples:

sample_1: Myristoylated Neuronal Calcium Sensor1, [U-100% 15N], 0.8 – 1 mM; H2O 90%; D2O 10%; Tris 50 mM; KCl 100 mM; DTT 1 mM

sample_conditions_1: ionic strength: 0.150 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1

Software:

CARA v1.8.4.2, Rochus Keller - data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

UNP P62166 P62168
BMRB 16334 4378
PDB
DBJ BAC37557 BAG73169
EMBL CAA57678 CAA58867 CAG05167 CAH90617 CAJ81731
GB AAA85633 AAA88510 AAC59690 AAD01642 AAF01804
REF NP_001018350 NP_001035637 NP_001084088 NP_001085136 NP_001122298
SP P62166 P62167 P62168 Q2V8Y7 Q5RC90
TPG DAA24173
AlphaFold Q9UK26 P62168 Q5RC90 P62167 P62166 Q2V8Y7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts