BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17181

Title: NMR Snapshots of a Fluctuating Protein Structure: Ubiquitin at 30bar-3kbar   PubMed: 15740740

Deposition date: 2010-09-10 Original release date: 2010-11-10

Authors: Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki

Citation: Kitahara, Ryo; Yokoyama, Shigeyuki; Akasaka, Kazuyuki. "NMR Snapshots of a Fluctuating Protein Structure: Ubiquitin at 30 bar-3 kbar"  J. Mol. Biol. 347, 277-285 (2005).

Assembly members:
ubiquitin, polymer, 76 residues, Formula weight is not available

Natural source:   Common Name: cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: obtained from a vendor

Entity Sequences (FASTA):
ubiquitin: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG

Data typeCount
kinetic rates4

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin1

Entities:

Entity 1, ubiquitin 76 residues - Formula weight is not available

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Samples:

sample_1: deuterated acetate buffer 30 mM; ubiquitin, [U-100% 15N], 3.0 mM; H2O 95%; D2O 95%

sample_conditions_1: pH: 4.6; pressure: 29.61 atm; temperature: 293 K

sample_conditions_2: pH: 4.6; pressure: 2960 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
NH-NOEsample_1isotropicsample_conditions_1
NH-NOEsample_1isotropicsample_conditions_2

Software:

ModelFree v4.15, Palmer - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis

NMR spectrometers:

  • Bruker DRX 800 MHz

Related Database Links:

BMRB 11505 11547 15047 15410 15689 15907 16228 16582 16626 16895 17439 17769 17919 18582 18583 18584 18610 18611 18737 19406 19412 25070 25123 25601 26604 4245 4375
PDB
DBJ BAA03983 BAA09860 BAA11842 BAA11843 BAA23486
EMBL CAA25706 CAA26488 CAA28495 CAA30183 CAA30815
GB AAA02769 AAA28154 AAA28997 AAA28998 AAA28999
PIR I50437 I51568 I65237 JN0790 S13928
PRF 0412265A 1101405A 1212243A 1212243B 1212243C
REF NP_001005123 NP_001006688 NP_001009117 NP_001009202 NP_001009286
SP P0C273 P0C275 P0C276 P0CG47 P0CG48
TPD FAA00319
TPE CEL68433 CEL70397 CEL75964 CEL78064
TPG DAA18802 DAA20663 DAA20672 DAA24675 DAA28295
AlphaFold P0C275 P0CG48 P0CG47 P0C273 P0C276