BMRB Entry 17373
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17373
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Title: PDZ2 from human SAP97 PubMed: 21113079
Deposition date: 2010-12-21 Original release date: 2011-01-14
Authors: Chi, Celestine; Bach, Anders; Engstrom, Ake; Stromgaard, Kristian; Lundstrom, Patrik; Ferguson, Neil; Jemth, Per
Citation: Chi, Celestine; Bach, Anders; Engstrom, Ake; Strmgaard, Kristian; Lundstrom, Patrik; Ferguson, Neil; Jemth, Per. "Biophysical Characterization of the Complex between Human Papillomavirus E6 Protein and Synapse-associated Protein 97." J. Biol. Chem. 286, 3597-3606 (2011).
Assembly members:
SAP97_PDZ2_polypeptide, polymer, 109 residues, 11678 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSET
Entity Sequences (FASTA):
SAP97_PDZ2_polypeptide: MHHHHHLVPRGSKPVSEKIM
EIKLIKGPKGLGFSIAGGVG
NQHIPGDNSIYVTKIIEGGA
AHKDGKLQIGDKLLAVNNVA
LEEVTHEEAVTALKNTSDFV
YLKVAKPTS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 277 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pdz domain | 1 |
Entities:
Entity 1, pdz domain 109 residues - 11678 Da.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | LEU | VAL | PRO | ARG | ||||
| 2 | GLY | SER | LYS | PRO | VAL | SER | GLU | LYS | ILE | MET | ||||
| 3 | GLU | ILE | LYS | LEU | ILE | LYS | GLY | PRO | LYS | GLY | ||||
| 4 | LEU | GLY | PHE | SER | ILE | ALA | GLY | GLY | VAL | GLY | ||||
| 5 | ASN | GLN | HIS | ILE | PRO | GLY | ASP | ASN | SER | ILE | ||||
| 6 | TYR | VAL | THR | LYS | ILE | ILE | GLU | GLY | GLY | ALA | ||||
| 7 | ALA | HIS | LYS | ASP | GLY | LYS | LEU | GLN | ILE | GLY | ||||
| 8 | ASP | LYS | LEU | LEU | ALA | VAL | ASN | ASN | VAL | ALA | ||||
| 9 | LEU | GLU | GLU | VAL | THR | HIS | GLU | GLU | ALA | VAL | ||||
| 10 | THR | ALA | LEU | LYS | ASN | THR | SER | ASP | PHE | VAL | ||||
| 11 | TYR | LEU | LYS | VAL | ALA | LYS | PRO | THR | SER |
Samples:
sample_1: SAP97 PDZ2 polypeptide, [U-100% 15N], 0.7 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 50 mM; pH: 6.9; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Varian INOVA 800 MHz
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts