BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17457

Title: The Solution Structure of Bovine Ferricytochrome b5 Determined Using Heteronuclear NMR Methods

Deposition date: 2011-02-11 Original release date: 2012-06-06

Authors: Muskett, Frederick; Kelly, Geoffrey; Whitford, David

Citation: Muskett, Frederick; Kelly, Geoffrey; Whitford, David. "The Solution Structure of Bovine Ferricytochrome b5 Determined Using Heteronuclear NMR Methods"  J. Mol. Biol. 258, 172-189 (1996).

Assembly members:
b5, polymer, 104 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:   Common Name: cattle   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK223-3

Entity Sequences (FASTA):
b5: AEESSKAVKYYTLEEIQKHN NSKSTWLILHYKVYDLTKFL EEHPGGEEVLREQAGGDATE NFEDVGHSTDARELSKTFII GELHPDDRSKITKPSESIIT TIDS

Data sets:
Data typeCount
kinetic rates1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1b51
2HEM2

Entities:

Entity 1, b5 104 residues - Formula weight is not available

1   ALAGLUGLUSERSERLYSALAVALLYSTYR
2   TYRTHRLEUGLUGLUILEGLNLYSHISASN
3   ASNSERLYSSERTHRTRPLEUILELEUHIS
4   TYRLYSVALTYRASPLEUTHRLYSPHELEU
5   GLUGLUHISPROGLYGLYGLUGLUVALLEU
6   ARGGLUGLNALAGLYGLYASPALATHRGLU
7   ASNPHEGLUASPVALGLYHISSERTHRASP
8   ALAARGGLULEUSERLYSTHRPHEILEILE
9   GLYGLULEUHISPROASPASPARGSERLYS
10   ILETHRLYSPROSERGLUSERILEILETHR
11   THRILEASPSER

Entity 2, HEM - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: b5, [U-15N], 2.4 mM; phosphate 20 mM; HEM 2.4 mM; D2O 100%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESY HSQCsample_1isotropicsample_conditions_1

Software:

FELIX v2.3, Accelrys Software Inc. - processing

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
BMRB 1323 1324
EMBL CAA31949
GB AAA72186 AAB32285 AAC14455 AAC48780 AAI08114
PRF 1106188A 1803548B
REF NP_001159663 NP_001274163 NP_776458 XP_005904370 XP_005972547
SP P00171
TPG DAA15965
AlphaFold P00171