BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17698

Title: HP67 H41F   PubMed: 21903098

Deposition date: 2011-06-12 Original release date: 2015-04-23

Authors: Brown, Jeffrey; Farelli, Jeremiah; McKnight, Christopher

Citation: Brown, Jeffrey; Farelli, Jeremiah; McKnight, Christopher. "On unsatisfied hydrogen bonds in the N-terminal subdomain of villin headpiece"  J. Mol. Biol. 413, 543-547 (2011).

Assembly members:
HP67_H41F, polymer, 67 residues, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: HP67_H41F

Entity Sequences (FASTA):
HP67_H41F: PTKLETFPLDVLVNTAAEDL PRGVDPSRKENFLSDEDFKA VFGMTRSAFANLPLWKQQNL KKEKGLF

Data typeCount
15N chemical shifts67
1H chemical shifts73
heteronuclear NOE values219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HP67 H41F1

Entities:

Entity 1, HP67 H41F 67 residues - Formula weight is not available

1   PROTHRLYSLEUGLUTHRPHEPROLEUASP
2   VALLEUVALASNTHRALAALAGLUASPLEU
3   PROARGGLYVALASPPROSERARGLYSGLU
4   ASNPHELEUSERASPGLUASPPHELYSALA
5   VALPHEGLYMETTHRARGSERALAPHEALA
6   ASNLEUPROLEUTRPLYSGLNGLNASNLEU
7   LYSLYSGLULYSGLYLEUPHE

Samples:

sample_1: HP67 H41F, [U-99% 15N], 1.4 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.01 M; pH: 7.0; pressure: 1 atm; temperature: 277 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts