BMRB Entry 4302
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4302
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Title: Assignment of 1H, 13C and 15N Resonances of the a' Domain of Protein Disulfide Isomerase
Deposition date: 1999-01-28 Original release date: 1999-08-12
Authors: Dijkstra, Klaas; Karvonen, Paeivi; Huovinen, Annamari; Koivunen, Peppi; Kivirikko, Kari; Darby, Nigel; van Straaten, Monique; Scheek, Ruud; Kemmink, Johan
Citation: Dijkstra, Klaas; Karvonen, Paeivi; Huovinen, Annamari; Koivunen, Peppi; Kivirikko, Kari; Darby, Nigel; van Straaten, Monique; Scheek, Ruud; Kemmink, Johan. "Assignment of 1H, 13C and 15N Resonances of the a' Domain of Protein Disulfide Isomerase" J. Biomol. NMR ., .-..
Assembly members:
Protein Dilsulfide Isomerase a' domain, polymer, 115 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET12a
Entity Sequences (FASTA):
Protein Dilsulfide Isomerase a' domain: DKQPVKVLVGKNFEDVAFDE
KKNVFVEFYAPWCGHCKQLA
PIWDKLGETYKDHENIVIAK
MDSTANEVEAVKVHSFPTLK
FFPASADRTVIDYNGERTLD
GFKKFLESGGQDGAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 519 |
| 15N chemical shifts | 114 |
| 1H chemical shifts | 778 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PDI a' | 1 |
Entities:
Entity 1, PDI a' 115 residues - Formula weight is not available
| 1 | ASP | LYS | GLN | PRO | VAL | LYS | VAL | LEU | VAL | GLY | ||||
| 2 | LYS | ASN | PHE | GLU | ASP | VAL | ALA | PHE | ASP | GLU | ||||
| 3 | LYS | LYS | ASN | VAL | PHE | VAL | GLU | PHE | TYR | ALA | ||||
| 4 | PRO | TRP | CYS | GLY | HIS | CYS | LYS | GLN | LEU | ALA | ||||
| 5 | PRO | ILE | TRP | ASP | LYS | LEU | GLY | GLU | THR | TYR | ||||
| 6 | LYS | ASP | HIS | GLU | ASN | ILE | VAL | ILE | ALA | LYS | ||||
| 7 | MET | ASP | SER | THR | ALA | ASN | GLU | VAL | GLU | ALA | ||||
| 8 | VAL | LYS | VAL | HIS | SER | PHE | PRO | THR | LEU | LYS | ||||
| 9 | PHE | PHE | PRO | ALA | SER | ALA | ASP | ARG | THR | VAL | ||||
| 10 | ILE | ASP | TYR | ASN | GLY | GLU | ARG | THR | LEU | ASP | ||||
| 11 | GLY | PHE | LYS | LYS | PHE | LEU | GLU | SER | GLY | GLY | ||||
| 12 | GLN | ASP | GLY | ALA | GLY |
Samples:
sample_1: Protein Dilsulfide Isomerase a' domain, [U-13C], 1.0 – 2.0 mM
sample_2: Protein Dilsulfide Isomerase a' domain, [U-13C; U-15N], 1.0 – 2.0 mM
conditions_1: pH: 6.8 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| BMRB | 11114 |
| PDB | |
| DBJ | BAE39791 BAG53171 BAG54732 |
| EMBL | CAA30112 |
| GB | AAH14504 AIC63098 EAW89691 EAW89694 EAW89696 |
| REF | XP_008011468 XP_011850754 XP_011850814 XP_012306507 |
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