BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4831

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Lysozyme   PubMed: 11274458

Deposition date: 2000-09-15 Original release date: 2001-05-01

Authors: Schwalbe, Harald; Grimshaw, Shaun; Spencer, Andrew; Buck, Matthias; Boyd, Jonathan; Dobson, Christopher; Redfield, Christina; Smith, Lorna

Citation: Schwalbe, Harald; Grimshaw, Shaun; Spencer, Andrew; Buck, Matthias; Boyd, Jonathan; Dobson, Christopher; Redfield, Christina; Smith, Lorna. "A Refined Solution Structure of Hen Lysozyme Determined Using Residual Dipolar Coupling Data"  Protein Sci. 10, 677-688 (2001).

Assembly members:
hen egg white lysozyme, polymer, 129 residues, Formula weight is not available

Natural source:   Common Name: hen   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
hen egg white lysozyme: KVFGRCELAAAMKRHGLDNY RGYSLGNWVCAAKFESNFNT EATNRNTDGSTDYGILQINS RWWCNDGRTPGSRNLCNIPC SALLSSDITASVNCAKKIVS DGNGMNAWVAWRNRCKGTDV QAWIRGCRL

Data typeCount
13C chemical shifts364
15N chemical shifts147
residual dipolar couplings209

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1lysozyme1

Entities:

Entity 1, lysozyme 129 residues - Formula weight is not available

1   LYSVALPHEGLYARGCYSGLULEUALAALA
2   ALAMETLYSARGHISGLYLEUASPASNTYR
3   ARGGLYTYRSERLEUGLYASNTRPVALCYS
4   ALAALALYSPHEGLUSERASNPHEASNTHR
5   GLUALATHRASNARGASNTHRASPGLYSER
6   THRASPTYRGLYILELEUGLNILEASNSER
7   ARGTRPTRPCYSASNASPGLYARGTHRPRO
8   GLYSERARGASNLEUCYSASNILEPROCYS
9   SERALALEULEUSERSERASPILETHRALA
10   SERVALASNCYSALALYSLYSILEVALSER
11   ASPGLYASNGLYMETASNALATRPVALALA
12   TRPARGASNARGCYSLYSGLYTHRASPVAL
13   GLNALATRPILEARGGLYCYSARGLEU

Samples:

sample_1: hen egg white lysozyme, [U-95% 13C; U-98% 15N], 1.5 mM

sample_2: hen egg white lysozyme, [U-15N], 0.5 mM; DMPC/DHPC 5%; phosphate 10 mM; H2O 93%; D2O 7%

sample_3: hen egg white lysozyme, [U-15N], 0.5 mM; DMPC/DHPC/CTAB 7.5%; phosphate 10 mM; H2O 93%; D2O 7%

ex-cond_1: pH: 3.8; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot availableex-cond_1
1H-15N TOCSY-HSQCnot availablenot availableex-cond_1
1H-15N HSQC-NOESY-HSQCnot availablenot availableex-cond_1
HNCOnot availablenot availableex-cond_1
HNCAnot availablenot availableex-cond_1
CBCACONHnot availablenot availableex-cond_1
CBCANHnot availablenot availableex-cond_1
HCCH-TOCSYnot availablenot availableex-cond_1
HCCH-NOESYnot availablenot availableex-cond_1
SOFT HCCH-COSYnot availablenot availableex-cond_1
SOFT HCCH-E.COSYnot availablenot availableex-cond_1
HMQCJnot availablenot availableex-cond_1

Software:

FELIX v98 - Assignment

NMR spectrometers:

  • Omega . 750 MHz
  • Omega . 600 MHz
  • Omega . 500 MHz
  • Bruker . 800 MHz
  • Bruker . 600 MHz