BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4930

Title: 1H and 15N Chemical Shift Assignments for the homodimer of human TFF1   PubMed: 11286998

Deposition date: 2000-12-22 Original release date: 2001-01-04

Authors: Williams, Mark; Westley, Bruce; May, Felicity; Feeney, James

Citation: Williams, Mark; Westley, Bruce; May, Felicity; Feeney, James. "The Solution Structure of the Disulphide-linked Homodimer of the Human Trefoil Protein TFF1"  FEBS Lett. 493, 70-74 (2001).

Assembly members:
Trefoil Factor Family 1, polymer, 60 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pezz18 (Pharmacia)

Entity Sequences (FASTA):
Trefoil Factor Family 1: EAQTETCTVAPRERQNCGFP GVTPSQCANKGCCFDDTVRG VPWCFYPNTIDVPPEEECEF

Data sets:
Data typeCount
15N chemical shifts59
1H chemical shifts386

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit 11
2subunit 21

Entities:

Entity 1, subunit 1 60 residues - Formula weight is not available

1   GLUALAGLNTHRGLUTHRCYSTHRVALALA
2   PROARGGLUARGGLNASNCYSGLYPHEPRO
3   GLYVALTHRPROSERGLNCYSALAASNLYS
4   GLYCYSCYSPHEASPASPTHRVALARGGLY
5   VALPROTRPCYSPHETYRPROASNTHRILE
6   ASPVALPROPROGLUGLUGLUCYSGLUPHE

Samples:

sample_1: Trefoil Factor Family 1, U-95% 15N, 2 mM; sodium phosphate 5 mM

cond_1: ionic strength: 0.01 M; pH: 5.9; temperature: 298 K

cond_2: ionic strength: 0.01 M; pH: 5.9; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
ROESYnot availablenot availablenot available
15N-1H HSQCnot availablenot availablenot available
15N-1H HSQC-NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY 600 MHz
  • Varian UNITY PLUS 500 MHz

Related Database Links:

BMRB 4933
PDB
DBJ BAA95532 BAB13729
EMBL CAA25155 CAA28695 CAA36254
GenBank AAA52402 AAH32811 AAX36234 AAX36702 AAX41076
PRF 1502207A 1716375A
REF NP_003216
SWISS-PROT P04155

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts