BMRB Entry 4931
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4931
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Title: Solution structure of dynein light chain 8 (DLC8) and bim peptide complex PubMed: 11178896
Deposition date: 2000-12-21 Original release date: 2001-08-22
Authors: Fan, Jing-Song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie
Citation: Fan, Jing-song.; Zhang, Qiang; Tochio, Hidehito; Li, Ming; Zhang, Mingjie. "Structural Basis of Diverse Sequence-dependent Target Recognition by the 8 kDa Dynein Light Chain " J. Mol. Biol. 306, 97-108 (2001).
Assembly members:
DYNEIN light chain 8, polymer, 89 residues, 10300 Da.
Bcl-2, polymer, 9 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET14B
Entity Sequences (FASTA):
DYNEIN light chain 8: MCDRKAVIKNADMSEEMQQD
SVECATQALEKYNIEKDIAA
HIKKEFDKKYNPTWHCIVGR
NFGSYVTHETKHFIYFYLGQ
VAILLFKSG
Bcl-2: MSCDKSTQT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 608 |
| 13C chemical shifts | 259 |
| 15N chemical shifts | 89 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | dynein unit 1 | 1 |
| 2 | dynein unit 2 | 1 |
| 3 | Bim unit 1 | 2 |
| 4 | Bim unit 2 | 2 |
Entities:
Entity 1, dynein unit 1 89 residues - 10300 Da.
| 1 | MET | CYS | ASP | ARG | LYS | ALA | VAL | ILE | LYS | ASN | ||||
| 2 | ALA | ASP | MET | SER | GLU | GLU | MET | GLN | GLN | ASP | ||||
| 3 | SER | VAL | GLU | CYS | ALA | THR | GLN | ALA | LEU | GLU | ||||
| 4 | LYS | TYR | ASN | ILE | GLU | LYS | ASP | ILE | ALA | ALA | ||||
| 5 | HIS | ILE | LYS | LYS | GLU | PHE | ASP | LYS | LYS | TYR | ||||
| 6 | ASN | PRO | THR | TRP | HIS | CYS | ILE | VAL | GLY | ARG | ||||
| 7 | ASN | PHE | GLY | SER | TYR | VAL | THR | HIS | GLU | THR | ||||
| 8 | LYS | HIS | PHE | ILE | TYR | PHE | TYR | LEU | GLY | GLN | ||||
| 9 | VAL | ALA | ILE | LEU | LEU | PHE | LYS | SER | GLY |
Entity 2, Bim unit 1 9 residues - Formula weight is not available
| 1 | MET | SER | CYS | ASP | LYS | SER | THR | GLN | THR |
Samples:
sample_1: DYNEIN light chain 8, [U-15N; U-13C], 1.5 mM; Bcl-2 1.5 mM
sample_cond_1: pH: 7.0; temperature: 303 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N-separated NOESY | not available | not available | not available |
| 3D 13C-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
VNMR v6.1b - data collection
NMRPipe v1.7 - data processing
X-PLOR v3.8 - structure solution
CNS v1.0 - refinement
NMR spectrometers:
- Varian INOVA 750 MHz
Related Database Links:
| SWISS-PROT | P63169 P63168 P63167 P61285 P61273 |
| REF | NP_003737 NP_001075487 NP_001032584 NP_001032583 NP_001003901 |
| GenBank | AAD01643 AAC32531 AAC32530 AAB38257 AAB04149 |
| EMBL | CAG46925 CAG28600 |
| DBJ | BAB28973 BAB28970 BAB27117 BAB27063 BAB22160 |
| PDB | |
| BMRB | 4912 4911 4305 |
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