BMRB Entry 5064
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5064
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Title: Backbone 1H, 13C, and 15N resonance assignments for a 14 kD protein, GABAA receptor associated protein (GABARAP)
Deposition date: 2001-06-29 Original release date: 2001-11-06
Authors: Harris, Richard; McAlister, Mark; Sankar, Andrew; Moss, Stephen; Keep, Nicholas; Driscoll, Paul
Citation: Harris, Richard; McAlister, Mark; Sankar, Andrew; Phelan, John; Moss, Stephen; Keep, Nicholas; Driscoll, Paul. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments for a 14 kD protein, GABAA receptor associated protein (GABARAP)" J. Biomol. NMR 21, 185-186 (2001).
Assembly members:
GABA(A) receptor associated protein, polymer, 136 residues, 15998 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
GABA(A) receptor associated protein: MGSSHHHHHHSSGLVPRGSM
KFVYKEEHPFEKRRSEGEKI
RKKYPDRVPVIVEKAPKARI
GDLDKKKYLVPSDLTVGQFY
FLIRKRIHLRAEDALFFFVN
NVIPPTSATMGQLYQEHHEE
DFFLYIAYSDESVYGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 441 |
| 13C chemical shifts | 343 |
| 15N chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GABARAP | 1 |
Entities:
Entity 1, GABARAP 136 residues - 15998 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | MET | ||||
| 3 | LYS | PHE | VAL | TYR | LYS | GLU | GLU | HIS | PRO | PHE | ||||
| 4 | GLU | LYS | ARG | ARG | SER | GLU | GLY | GLU | LYS | ILE | ||||
| 5 | ARG | LYS | LYS | TYR | PRO | ASP | ARG | VAL | PRO | VAL | ||||
| 6 | ILE | VAL | GLU | LYS | ALA | PRO | LYS | ALA | ARG | ILE | ||||
| 7 | GLY | ASP | LEU | ASP | LYS | LYS | LYS | TYR | LEU | VAL | ||||
| 8 | PRO | SER | ASP | LEU | THR | VAL | GLY | GLN | PHE | TYR | ||||
| 9 | PHE | LEU | ILE | ARG | LYS | ARG | ILE | HIS | LEU | ARG | ||||
| 10 | ALA | GLU | ASP | ALA | LEU | PHE | PHE | PHE | VAL | ASN | ||||
| 11 | ASN | VAL | ILE | PRO | PRO | THR | SER | ALA | THR | MET | ||||
| 12 | GLY | GLN | LEU | TYR | GLN | GLU | HIS | HIS | GLU | GLU | ||||
| 13 | ASP | PHE | PHE | LEU | TYR | ILE | ALA | TYR | SER | ASP | ||||
| 14 | GLU | SER | VAL | TYR | GLY | LEU |
Samples:
Sample_1: GABA(A) receptor associated protein, [U-13C; U-15N], 0.5 – 1.0 mM
sample_conditions: pH: 7.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-13C HCCH-TOCSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
Software:
nmrPIPE - data processing
ANSIG vv3.3 - spectral analysis
NMR spectrometers:
- Varian UNITYplus 500 MHz
- Varian UNITYplus 600 MHz
Related Database Links:
| BMRB | 5058 5128 |
| PDB | |
| DBJ | BAB21549 BAB22426 BAB27806 BAG35138 |
| EMBL | CAG00707 CAG33324 CAG47031 CAP17839 CDQ58630 |
| GB | AAD02337 AAD32455 AAD47641 AAD47642 AAD47643 |
| REF | NP_001011192 NP_001013278 NP_001029220 NP_001075611 NP_001084530 |
| SP | O95166 P60517 Q8MK68 Q9DCD6 Q9GJW7 |
| TPG | DAA18837 |
| AlphaFold | P60517 Q8MK68 Q9DCD6 O95166 Q9GJW7 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts