BMRB Entry 5361
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5361
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NMR-STAR v3 text file.
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Title: Solution Structure of the DNA Complex of Human TRF1 PubMed: 11738049
Deposition date: 2001-05-03 Original release date: 2002-06-26
Authors: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.
Citation: Nishikawa, T.; Okamura, H.; Nagadoi, A.; Konig, P.; Rhodes, D.; Nishimura, Y.. "Solution Structure of a Telomeric DNA Complex of Human TRF1" Structure 9, 1237-1251 (2001).
Assembly members:
Telomeric repeat binding factor 1, polymer, 70 residues, Formula weight is not available
none, polymer, 13 residues, Formula weight is not available
none, polymer, 13 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Telomeric repeat binding factor 1: MTPEKHRARKRQAWLWEEDK
NLRSGVRKYGEGNWSKILLH
YKFNNRTSVMLKDRWRTMKK
LKLISSDSED
none: GTTAGGGTTAGGG
none: CCCTAACCCTAAC
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 655 |
13C chemical shifts | 186 |
15N chemical shifts | 67 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TELOMERE REPEAT BINDING FACTOR 1 | 1 |
2 | 5'-D(*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3' | 2 |
3 | 5'-D(*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*C)-3' | 3 |
Entities:
Entity 1, TELOMERE REPEAT BINDING FACTOR 1 70 residues - Formula weight is not available
1 | MET | THR | PRO | GLU | LYS | HIS | ARG | ALA | ARG | LYS | |
2 | ARG | GLN | ALA | TRP | LEU | TRP | GLU | GLU | ASP | LYS | |
3 | ASN | LEU | ARG | SER | GLY | VAL | ARG | LYS | TYR | GLY | |
4 | GLU | GLY | ASN | TRP | SER | LYS | ILE | LEU | LEU | HIS | |
5 | TYR | LYS | PHE | ASN | ASN | ARG | THR | SER | VAL | MET | |
6 | LEU | LYS | ASP | ARG | TRP | ARG | THR | MET | LYS | LYS | |
7 | LEU | LYS | LEU | ILE | SER | SER | ASP | SER | GLU | ASP |
Entity 2, 5'-D(*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3' 13 residues - Formula weight is not available
1 | DG | DT | DT | DA | DG | DG | DG | DT | DT | DA | ||||
2 | DG | DG | DG |
Entity 3, 5'-D(*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*AP*AP*C)-3' 13 residues - Formula weight is not available
1 | DC | DC | DC | DT | DA | DA | DC | DC | DC | DT | ||||
2 | DA | DA | DC |
Samples:
sample_1: Telomeric repeat binding factor 1, [U-15N; U-13C], 1.5 2.5 mM; none1.5 2.5 mM; none1.5 2.5 mM; Phosphate buffer 5 mM; NaCl 10 mM
sample_cond_1: pH: 6.8; temperature: 305 K; pressure: 1 atm
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | sample_1 | not available | sample_cond_1 |
3D HN(CO)CA | sample_1 | not available | sample_cond_1 |
3D CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
3D C(CO)NH | sample_1 | not available | sample_cond_1 |
3D HBHA(CO)NH | sample_1 | not available | sample_cond_1 |
3D HCCH-TOCSY | sample_1 | not available | sample_cond_1 |
3D HNHA | sample_1 | not available | sample_cond_1 |
3D HNHB | sample_1 | not available | sample_cond_1 |
3D 13C-NOESY | sample_1 | not available | sample_cond_1 |
3D 15N-NOESY | sample_1 | not available | sample_cond_1 |
2D NOESY | sample_1 | not available | sample_cond_1 |
2D TOCSY | sample_1 | not available | sample_cond_1 |
2D COSY with or without isotope filtering | sample_1 | not available | sample_cond_1 |
Software:
EMBOSS v5.0 - refinement, structure solution
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 500 MHz
- Bruker DMX 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts