BMRB Entry 6414
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6414
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Title: FRAGMENT 33-61 OF BOVINE alpha-HEMOGLBIN: THE EFFECT OF C-TERMINAL AMIDATION AND IDENTIFICATION OF THE MINIMAL PORTION WITH ANTIFUNGAL ACTIVITY PubMed: 15850378
Deposition date: 2004-12-06 Original release date: 2008-07-17
Authors: Machado, Alessandra; Sforca, Mauricio; Miranda, Antonio; Daffre, Sirlei; Spisni, Alberto; Pertinhez, Thelma; Miranda, M. Teresa
Citation: Sforca, Mauricio; Machado, Alessandra; Figueredo, Rita; Oyama, Sergio; Silva, Fernanda; Miranda, Antonio; Daffre, Sirlei; Miranda, M.; Spisni, Alberto; Pertinhez, Thelma. "The micelle-bound structure of an antimicrobial peptide derived from the alpha-chain of bovine hemoglobin isolated from the tick Boophilus microplus." Biochemistry 44, 6440-6451 (2005).
Assembly members:
amidated fragment 33-52, polymer, 21 residues, 2327.6 Da.
Natural source: Common Name: Cow Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
amidated fragment 33-52: FLSFPTTKTYFPHFDLSHGS
X
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | amidated fragment 33-52 | 1 |
Entities:
Entity 1, amidated fragment 33-52 21 residues - 2327.6 Da.
| 1 | PHE | LEU | SER | PHE | PRO | THR | THR | LYS | THR | TYR | ||||
| 2 | PHE | PRO | HIS | PHE | ASP | LEU | SER | HIS | GLY | SER | ||||
| 3 | NH2 |
Samples:
sample_1: amidated fragment 33-52 1.0 mM; sodium dodecyl sulfate (SDS) 200 mM
Ex-cond_1: pH: 4.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H DQF-COSY | sample_1 | not available | Ex-cond_1 |
| NOESY | sample_1 | not available | Ex-cond_1 |
| ROESY | sample_1 | not available | Ex-cond_1 |
| TOCSY | sample_1 | not available | Ex-cond_1 |
Software:
NMRrpipe_NMRview v5.0 -
NMR spectrometers:
- Varian Inova 500 MHz
Related Database Links:
| BMRB | 1101 16891 16898 25 2707 2709 2868 3442 5809 6048 907 |
| PDB | |
| DBJ | BAD97112 BAJ20323 |
| EMBL | CAA23748 CAA23750 CAA23752 CAA23774 CAA25044 |
| GB | AAA30909 AAA30910 AAA35449 AAA36844 AAA52631 |
| PIR | A24693 HAMQR |
| PRF | 0404170A 0409309A 0504211A 0907233A 1004268B |
| REF | NP_000508 NP_000549 NP_001036091 NP_001036092 NP_001038189 |
| SP | B3EWC7 B3EWC9 P01923 P01924 P01926 |
| TPG | DAA15749 |
| AlphaFold | B3EWC9 P01923 P01924 P01926 B3EWC7 |