BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6521

Title: Characterization of an amyloid fibril intermediate   PubMed: 16260757

Deposition date: 2005-02-22 Original release date: 2007-01-29

Authors: Galea, Charles; Bowman, Prentice; Kriwacki, Richard

Citation: Galea, Charles; Bowman, Prentice; Kriwacki, Richard. "Disruption of an intermonomer salt bridge in the p53 tetramerization domain results in an increased propensity to form amyloid fibrils."  Protein Sci. 14, 2993-3003 (2005).

Assembly members:
p53tet, polymer, 55 residues, 6265 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):
p53tet: GSHMNNTSSSPQPKKKPLDG EYFTLQIRGRERFEMFRELN EALELKDAQAGKEPG

Data sets:
Data typeCount
1H chemical shifts98
15N chemical shifts98
13C chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1
4subunit D1

Entities:

Entity 1, subunit A 55 residues - 6265 Da.

Residues 10 to 60 correspond to the residues 310 to 360 of the p53 tetramerization domain.

1   GLYSERHISMETASNASNTHRSERSERSER
2   PROGLNPROLYSLYSLYSPROLEUASPGLY
3   GLUTYRPHETHRLEUGLNILEARGGLYARG
4   GLUARGPHEGLUMETPHEARGGLULEUASN
5   GLUALALEUGLULEULYSASPALAGLNALA
6   GLYLYSGLUPROGLY

Samples:

sample_3: p53tet, [U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%

sample_1: p53tet, [U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%

sample_2: p53tet, [U-13C; U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%

sample_4: p53tet, [U-13C; U-15N], 4.0 mM; Na2HPO4 10 mM; NaCl 50 mM; NaN3 0.02%

conditions_1: pH: 6.0; temperature: 293.0 K

conditions_2: pH: 4.0; temperature: 293.0 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
1H15N_NOESYnot availablenot availablenot available

Software:

Felix, Accelrys -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 4048 6522 7251
PDB
DBJ BAC16799 BAD96746 BAG35463 BAG59884 BAG60244
EMBL CAA25652 CAA26306 CAA34420 CAA38095 CAA42626
GB AAA17994 AAA59987 AAA59988 AAA59989 AAA61211
PRF 2006287A
REF NP_000537 NP_001040616 NP_001119584 NP_001119587 NP_001119590
SP P04637 P13481 P56423 P56424 P61260
AlphaFold P13481 P04637 P56423 P56424 P61260

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts