BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6552

Title: A Protein Folding Pathway with Multiple Folding Intermediates at Atomic Resolution   PubMed: 15793003

Deposition date: 2005-03-18 Original release date: 2005-08-30

Authors: Feng, H.; Zhou, Z.; Bai, Y.

Citation: Feng, H.; Zhou, Z.; Bai, Y.. "A protein folding pathway with multiple folding intermediates at atomic resolution"  Proc. Natl. Acad. Sci. U. S. A. 102, 5026-5031 (2005).

Assembly members:
redesigned apo-cytochrome b562, polymer, 106 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homon sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
redesigned apo-cytochrome b562: ADLEDNDETGNDNGKGGEKA DNAAQVKDALTKMRAAALDA QKATPPKLEDKSPDSPEMKD FRHGFDILVGQIDDALKLAN EGKVKEAQAAAEQLKTTGRA GNQKGG

Data sets:
Data typeCount
13C chemical shifts311
15N chemical shifts116
1H chemical shifts686
coupling constants100

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1redesigned apo-cytochrome b5621

Entities:

Entity 1, redesigned apo-cytochrome b562 106 residues - Formula weight is not available

1   ALAASPLEUGLUASPASNASPGLUTHRGLY
2   ASNASPASNGLYLYSGLYGLYGLULYSALA
3   ASPASNALAALAGLNVALLYSASPALALEU
4   THRLYSMETARGALAALAALALEUASPALA
5   GLNLYSALATHRPROPROLYSLEUGLUASP
6   LYSSERPROASPSERPROGLUMETLYSASP
7   PHEARGHISGLYPHEASPILELEUVALGLY
8   GLNILEASPASPALALEULYSLEUALAASN
9   GLUGLYLYSVALLYSGLUALAGLNALAALA
10   ALAGLUGLNLEULYSTHRTHRGLYARGALA
11   GLYASNGLNLYSGLYGLY

Samples:

sample_1: redesigned apo-cytochrome b562 mM; sodium acetate 50 mM; H2O 92.5%; D2O 7.5%

sample_2: redesigned apo-cytochrome b562 mM; sodium acetate 50 mM; D2O 99%

sample_cond_1: pH: 4.84; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D HSQCnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCACONHnot availablenot availablenot available
3D HAHBCONHnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
2DNOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

NMRPipe v5.0.4 - processing

X-PLOR vNIH-2.9 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 6553
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts