BMRB Entry 6616
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6616
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: 1H chemical shift assignments for peptide P5 PubMed: 16460023
Deposition date: 2005-05-02 Original release date: 2006-08-07
Authors: Martins, Rafael; Sforca, Mauricio; Amino, Rogerio; Juliano, Maria; Juliano, Luiz; Pertinhez, Thelma; Spisni, Alberto; Schenkman, Sergio
Citation: Martins, Rafael; Sforca, Mauricio; Amino, Rogerio; Juliano, Maria; Oyama, Sergio; Juliano, Luiz; Pertinhez, Thelma; Spisni, Alberto; Schenkman, Sergio. "Lytic Activity and Structural Differences of Amphipathic Peptides Derived from Trialysin" Biochemistry 45, 1765-1774 (2006).
Assembly members:
P5, polymer, 27 residues, 2896.6 Da.
Natural source: Common Name: Triatoma infestans Taxonomy ID: 30076 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Triatoma infestans
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
P5: GKVLDKFGKIVGKVLKQLKK
VSAVAKV
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 162 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | peptide P5 | 1 |
Entities:
Entity 1, peptide P5 27 residues - 2896.6 Da.
1 | GLY | LYS | VAL | LEU | ASP | LYS | PHE | GLY | LYS | ILE | ||||
2 | VAL | GLY | LYS | VAL | LEU | LYS | GLN | LEU | LYS | LYS | ||||
3 | VAL | SER | ALA | VAL | ALA | LYS | VAL |
Samples:
sample_1: P5 1.2 mM; TFE 30 % (vol); D2O 5 % (vol); H2O 65 % (vol)
P5_conditions: pH: 4; temperature: 310 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H TOCSY | sample_1 | not available | P5_conditions |
1H COSY | sample_1 | not available | P5_conditions |
1H NOESY | sample_1 | not available | P5_conditions |
1H ROESY | sample_1 | not available | P5_conditions |
Software:
NMRView5 v5, One Moon Scientific Inc. - chemical shift assignment, spectrum analysis
NMR spectrometers:
- Varian Unity 500 MHz