BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6667

Title: 15N hyperfine shifts assignment for rubredoxin (V8I) from Clostridium pasteurianum in the oxidized state

Deposition date: 2005-06-08 Original release date: 2005-12-20

Authors: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John

Citation: Lin, I-Jin; Gebel, Erika; Machonkin, Timothy; Westler, William; Markley, John. "Changes in hydrogen-bond strengths explain reduction potentials in 10 rubredoxin variants."  Proc. Natl. Acad. Sci. U. S. A. 11, 14581-14586 (2005).

Assembly members:
rubredoxin, polymer, 54 residues, Formula weight is not available
FE, non-polymer, 55.845 Da.

Natural source:   Common Name: Clostridium pasteurianum   Taxonomy ID: 1501   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Clostridium pasteurianum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
rubredoxin: MKKYTCTICGYIYNPEDGDP DNGVNPGTDFKDIPDDWVCP LCGVGKDQFEEVEE

Data sets:
Data typeCount
15N chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin (V8I)1
2FE (III) ION2

Entities:

Entity 1, rubredoxin (V8I) 54 residues - Formula weight is not available

1   METLYSLYSTYRTHRCYSTHRILECYSGLY
2   TYRILETYRASNPROGLUASPGLYASPPRO
3   ASPASNGLYVALASNPROGLYTHRASPPHE
4   LYSASPILEPROASPASPTRPVALCYSPRO
5   LEUCYSGLYVALGLYLYSASPGLNPHEGLU
6   GLUVALGLUGLU

Entity 2, FE (III) ION - Fe - 55.845 Da.

1   FE

Samples:

oxidized_rubredoxin_(V8I): rubredoxin, [U-15], 4 – 8 mM; phospate buffer 50 mM

50mM_phospate_buffer_pH6: pH: 6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1D 15Noxidized_rubredoxin_(V8I)isotropic50mM_phospate_buffer_pH6

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 4050 4051 4066 4137 4182 4319 4320 5600 6659 6660 6661 6662 6663 6664 6668 6669 6670
PDB
GB AAA23279 AJA49845 AJA53833 ELP57804 KER11884
REF WP_003447684
SP P00268
AlphaFold P00268