BMRB Entry 6789
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6789
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Title: Backbone 1H- and 15N-amide chemical shifts of apo-, (Ca2+)1- and (Ca2+)2-Eq04Q TR2C of bovine calmodulin, at 301K and pH 6.0. PubMed: 9753463
Deposition date: 2005-08-24 Original release date: 2013-02-15
Authors: Evenas, Johan; Malmendal, Anders; Thulin, Eva; Carlstrom, Goran; Forsen, Sture
Citation: Evenas, Johan; Malmendal, Anders; Thulin, Eva; Carlstrom, Goran; Forsen, Sture. "Ca2+ binding and conformational changes in a calmodulin domain." Biochemistry 37, 13744-13754 (1998).
Assembly members:
TR2C, polymer, 73 residues, Formula weight is not available
Natural source: Common Name: Bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
TR2C: MKDTDSEEEIREAFRVFDKD
GNGYISAAELRHVMTNLGEK
LTDEEVDEMIREADIDGDGQ
VNYEQFVQMMTAK
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 71 |
| 1H chemical shifts | 71 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E104Q TR2C | 1 |
Entities:
Entity 1, E104Q TR2C 73 residues - Formula weight is not available
| 1 | MET | LYS | ASP | THR | ASP | SER | GLU | GLU | GLU | ILE | ||||
| 2 | ARG | GLU | ALA | PHE | ARG | VAL | PHE | ASP | LYS | ASP | ||||
| 3 | GLY | ASN | GLY | TYR | ILE | SER | ALA | ALA | GLU | LEU | ||||
| 4 | ARG | HIS | VAL | MET | THR | ASN | LEU | GLY | GLU | LYS | ||||
| 5 | LEU | THR | ASP | GLU | GLU | VAL | ASP | GLU | MET | ILE | ||||
| 6 | ARG | GLU | ALA | ASP | ILE | ASP | GLY | ASP | GLY | GLN | ||||
| 7 | VAL | ASN | TYR | GLU | GLN | PHE | VAL | GLN | MET | MET | ||||
| 8 | THR | ALA | LYS |
Samples:
sample_1: TR2C, [U-15N], 1.16 mM; NaN3 0.1 mM; KOH 0.1 mM; HCl 0.1 mM
conditions_1: pH: 6.0; temperature: 301 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H COSY DQ | sample_1 | not available | conditions_1 |
| 1H TOCSY | sample_1 | not available | conditions_1 |
| 1H NOESY | sample_1 | not available | conditions_1 |
| 2D 15N HSQC | sample_1 | not available | conditions_1 |
| 1H-15N HSQC | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian UNITY PLUS 600 MHz
Related Database Links:
| BMRB | 11440 16994 17881 17981 17982 17983 18084 18323 19376 25344 7016 7017 7190 |
| PDB | |
| DBJ | BAC39089 BAF45809 BAI66109 BAI66110 BAI66111 |
| EMBL | CAB51566 |
| GB | AAH10730 AAO17827 AAQ14324 AAX61134 AAY87961 |
| REF | XP_001687795 XP_001869425 XP_007442524 XP_010190430 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts