BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7188

Title: Im7, Immunity Protein of Colicin E7   PubMed: 17045607

Deposition date: 2006-06-21 Original release date: 2006-10-30

Authors: Le Duff, Cecile; Whittaker, Sara; Radford, Sheena; Moore, Geoffrey

Citation: Le Duff, Cecile; Whittaker, Sara; Radford, Sheena; Moore, Geoffrey. "Characterisation of the Conformational Properties of Urea-unfolded Im7: Implications for the Early Stages of Protein Folding"  J. Mol. Biol. 364, 824-835 (2006).

Assembly members:
Im7 native, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: Escherichia Coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Im7 native: MELKNSISDYTEAEFVQLLK EIEKENVAATDDVLDVLLEH FVKITEHPDGTDLIYYPSDN RDDSPEGIVKEIKEWRAANG KPGFKQG

Data sets:
Data typeCount
13C chemical shifts486
15N chemical shifts81
1H chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Im7 native1

Entities:

Entity 1, Im7 native 87 residues - Formula weight is not available

1   METGLULEULYSASNSERILESERASPTYR
2   THRGLUALAGLUPHEVALGLNLEULEULYS
3   GLUILEGLULYSGLUASNVALALAALATHR
4   ASPASPVALLEUASPVALLEULEUGLUHIS
5   PHEVALLYSILETHRGLUHISPROASPGLY
6   THRASPLEUILETYRTYRPROSERASPASN
7   ARGASPASPSERPROGLUGLYILEVALLYS
8   GLUILELYSGLUTRPARGALAALAASNGLY
9   LYSPROGLYPHELYSGLNGLY

Samples:

sample_1: Im7, [U-15N; U-13C], 1 mM; Phosphate buffer 20

sample_2: Im7, [U-15N; U-13C], 1 mM; Phosphate buffer 20 mM; Urea 6 M

conditions_1: pH: 7; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 15N-1H HSQCnot availablenot availableconditions_1
2D 15N-1H HSQC NH2 onlynot availablenot availableconditions_1
3D HNCO, 3D HNCAnot availablenot availableconditions_1
3D HNCACBnot availablenot availableconditions_1
3D CBCA(CO)NHnot availablenot availableconditions_1
3D H(CCO)NHnot availablenot availableconditions_1
3D HN(CA)COnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Unity Inova 499.865 MHz

Related Database Links:

BMRB 7316 7317
PDB
EMBL CAA45165 CDK50318
GB AAA23071 AIC79148 ELE46623 EOW15734 EQR11492
REF WP_001560791 WP_032277812 YP_009060494
SP Q03708
AlphaFold Q03708

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts