BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11121

Title: The solution structure of the second fibronectin type III domain of human Neogenin

Deposition date: 2010-03-31 Original release date: 2011-04-01

Authors: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.

Citation: Tochio, N.; Koshiba, S.; Inoue, M.; Kigawa, T.; Yokoyama, S.. "The solution structure of the second fibronectin type III domain of human Neogenin"  .

Assembly members:
fn3 domain, polymer, 116 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P041213-03

Entity Sequences (FASTA):
fn3 domain: GSSGSSGRVETQPEVQLPGP APNLRAYAASPTSITVTWET PVSGNGEIQNYKLYYMEKGT DKEQDVDVSSHSYTINGLKK YTEYSFRVVAYNKHGPGVST PDVAVRTLSDSGPSSG

Data sets:
Data typeCount
13C chemical shifts463
15N chemical shifts108
1H chemical shifts706

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1fn3 domain1

Entities:

Entity 1, fn3 domain 116 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYARGVALGLU
2   THRGLNPROGLUVALGLNLEUPROGLYPRO
3   ALAPROASNLEUARGALATYRALAALASER
4   PROTHRSERILETHRVALTHRTRPGLUTHR
5   PROVALSERGLYASNGLYGLUILEGLNASN
6   TYRLYSLEUTYRTYRMETGLULYSGLYTHR
7   ASPLYSGLUGLNASPVALASPVALSERSER
8   HISSERTYRTHRILEASNGLYLEULYSLYS
9   TYRTHRGLUTYRSERPHEARGVALVALALA
10   TYRASNLYSHISGLYPROGLYVALSERTHR
11   PROASPVALALAVALARGTHRLEUSERASP
12   SERGLYPROSERSERGLY

Samples:

sample_1: FN3 domain, [U-13C; U-15N], 1.9 mM; d-Tris HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYsample_1isotropiccondition_1
3D 13C-separated NOESYsample_1isotropiccondition_1

Software:

xwinnmr v3.5, Bruker - collection

NMRPipe v20031121, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9295, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - structure solution

NMR spectrometers:

  • Bruker AVANCE 900 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts