BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11199

Title: Solution structure of the PDZ domain of Enigma homologue protein

Deposition date: 2010-07-22 Original release date: 2011-07-21

Authors: Qin, X.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Tochio, N.; Kigawa, T.; Hayashi, F.; Yokoyama, S.. "Solution structure of the PDZ domain of Enigma homologue protein"  .

Assembly members:
PDZ domain, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P030203-44

Entity Sequences (FASTA):
PDZ domain: GSSGSSGSVSLVGPAPWGFR LQGGKDFNMPLTISSLKDGG KASQAHVRIGDVVLSIDGIS AQGMTHLEAQNKIKACTGSL NMTLQRASAAAKSEPVSSGP SSG

Data sets:
Data typeCount
13C chemical shifts407
15N chemical shifts106
1H chemical shifts657

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Enigma homologue protein1

Entities:

Entity 1, Enigma homologue protein 103 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYSERVALSER
2   LEUVALGLYPROALAPROTRPGLYPHEARG
3   LEUGLNGLYGLYLYSASPPHEASNMETPRO
4   LEUTHRILESERSERLEULYSASPGLYGLY
5   LYSALASERGLNALAHISVALARGILEGLY
6   ASPVALVALLEUSERILEASPGLYILESER
7   ALAGLNGLYMETTHRHISLEUGLUALAGLN
8   ASNLYSILELYSALACYSTHRGLYSERLEU
9   ASNMETTHRLEUGLNARGALASERALAALA
10   ALALYSSERGLUPROVALSERSERGLYPRO
11   SERSERGLY

Samples:

sample_1: PDZ domain, [U-13C; U-15N], 1.06 mM; PiNa 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20020425, Delaglio.F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.854, Kobayashi, N. - data analysis

CYANA v1.0.7, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA88828 BAB22633 BAB26304 BAE28279
GB ABB03726 ADI88504 ADI88505 ADI88506 ADI88507
REF NP_001177781 NP_001177782 NP_001177783 NP_001177784 NP_001177785
SP Q8CI51
AlphaFold Q8CI51

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts