BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 11238

Title: Solution structure of the C2H2-type zinc finger domain (699-729) from zinc finger protein 473

Deposition date: 2010-07-23 Original release date: 2011-08-03

Authors: Qin, X.; Hayashi, F.; Yokoyama, S.

Citation: Qin, X.; Hayashi, F.; Yokoyama, S.. "Solution structure of the C2H2-type zinc finger domain (699-729) from zinc finger protein 473"  .

Assembly members:
C2H2-type zinc finger, polymer, 44 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: cell free synthesis   Host organism: E. coli - cell free   Vector: P070115-32

Entity Sequences (FASTA):
C2H2-type zinc finger: GSSGSSGKKPLVCNECGKTF RQSSCLSKHQRIHSGEKPSG PSSG

Data sets:
Data typeCount
13C chemical shifts158
15N chemical shifts34
1H chemical shifts242

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1C2H2-type zinc finger1
2ZINC ION2

Entities:

Entity 1, C2H2-type zinc finger 44 residues - Formula weight is not available

1   GLYSERSERGLYSERSERGLYLYSLYSPRO
2   LEUVALCYSASNGLUCYSGLYLYSTHRPHE
3   ARGGLNSERSERCYSLEUSERLYSHISGLN
4   ARGILEHISSERGLYGLULYSPROSERGLY
5   PROSERSERGLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C2H2-type zinc finger, [U-13C; U-15N], 0.06 mM; d-Tris-HCl 20 mM; NaCl 100 mM; d-DTT 1 mM; NaN3 0.02%; ZnCl2 0.05 mM; IDA 1 mM; H2O 90%; D2O 10%

condition_1: ionic strength: 120 mM; pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYsample_1isotropiccondition_1
3D 15N-separated NOESYsample_1isotropiccondition_1

Software:

VNMR v6.1C, Varian - collection

NMRPipe v20030801, Delaglio, F. - processing

NMRView v5.0.4, Johnson, B.A. - data analysis

Kujira v0.9820, Kobayashi, N. - data analysis

CYANA v2.0.17, Guntert, P. - refinement, structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts