BMRB Entry 20085
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR20085
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Title: Solution structure of SFT-L1 PubMed: 21423169
Deposition date: 2009-07-21 Original release date: 2011-03-21
Authors: Mylne, Joshua; Daly, Norelle; Colgrave, Michelle; McCallum, Emily; Jones, Alun; Craik, David
Citation: Mylne, Joshua; Colgrave, Michelle; Daly, Norelle; Chanson, Aurelie; Elliott, Alysha; McCallum, Emily; Jones, Alun; Craik, David. "Albumins and their processing machinery are hijacked for cyclic peptides in sunflower" Nat. Chem. Biol. 7, 257-259 (2011).
Assembly members:
SFT-L1, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: sunflowers Taxonomy ID: 4232 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Helianthus Helianthus annuus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
SFT-L1: GCIEGSPVCFPD
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 68 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | SFT-L1 | 1 |
Entities:
Entity 1, SFT-L1 12 residues - Formula weight is not available
1 | GLY | CYS | ILE | GLU | GLY | SER | PRO | VAL | CYS | PHE | ||||
2 | PRO | ASP |
Samples:
sample_1: SFT-L1 1 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5; temperature: 290 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v1.1, Bruker Biospin - collection
XEASY, Bartels et al. - data analysis
CYANA, Guntert, Mumenthaler and Wuthrich - structure solution
CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz