BMRB Entry 21011
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR21011
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: LPS bound structure of Cysteine deleted TachyplesinI PubMed: 22464970
Deposition date: 2011-06-10 Original release date: 2018-12-19
Authors: Bhattacharjya, Surajit; Saravanan, Rathi
Citation: Saravanan, Rathi; Mohanram, Harini; Joshii, Mangeshi; Domadiai, Prerna N.; Torresi, Jaume; Ruedl, Christianei; Bhattacharjya, Surajit. "Structure, activity and interactions of the cysteine deleted analog of tachyplesin-1 with lipopolysaccharide micelle: Mechanistic insights into outer-membrane permeabilization and endotoxin neutralization." Biochim. Biophys. Acta 1818, 1613-1624 (2012).
Assembly members:
CDT, polymer, 13 residues, 1862.254 Da.
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
CDT: KWFRVYRGIYRRR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CDT | 1 |
Entities:
Entity 1, CDT 13 residues - 1862.254 Da.
| 1 | LYS | TRP | PHE | ARG | VAL | TYR | ARG | GLY | ILE | TYR | ||||
| 2 | ARG | ARG | ARG |
Samples:
sample_1: CDT 500 uM; LPS 10 uM; H2O 90%; D2O 10%
sample_conditions_1: pH: 4.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - refinement
NMR spectrometers:
- Bruker AMX 600 MHz