BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25084

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sp Cdc5-D3   PubMed: 25263959

Deposition date: 2014-07-10 Original release date: 2019-07-11

Authors: Collier, Scott

Citation: Collier, Scott; Voehler, Markus; Peng, Dungeng; Ohi, Ryomi; Gould, Kathleen; Reiter, Nicholas; Ohi, Melanie. "Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5"  Biochemistry 53, 6439-6451 (2014).

Assembly members:
Cdc5-D3_(aa_155-214), polymer, 63 residues, 7471.6 Da.

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15-b

Entity Sequences (FASTA):
Cdc5-D3_(aa_155-214): GSHMDEDEKEMLSEARARLA NTQGKKAKRKDREKQLELTR RLSHLQKRRELKAAGINIKL FRR

Data sets:
Data typeCount
13C chemical shifts184
15N chemical shifts60
1H chemical shifts60

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cdc5-D3 monomer1

Entities:

Entity 1, Cdc5-D3 monomer 63 residues - 7471.6 Da.

1   GLYSERHISMETASPGLUASPGLULYSGLU
2   METLEUSERGLUALAARGALAARGLEUALA
3   ASNTHRGLNGLYLYSLYSALALYSARGLYS
4   ASPARGGLULYSGLNLEUGLULEUTHRARG
5   ARGLEUSERHISLEUGLNLYSARGARGGLU
6   LEULYSALAALAGLYILEASNILELYSLEU
7   PHEARGARG

Samples:

Cdc5-D3_(aa_155-214)_sample: Cdc5-D3 (aa 155-214), [U-99% 13C; U-99% 15N], 370 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCCdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D HNCOCdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D HNCACdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D HNCACBCdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D HN(CO)CACdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D CBCA(CO)NHCdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1
3D HN(CA)COCdc5-D3_(aa_155-214)_sampleanisotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

UNP P39964
AlphaFold P39964

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts