BMRB Entry 25084
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25084
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sp Cdc5-D3 PubMed: 25263959
Deposition date: 2014-07-10 Original release date: 2019-07-11
Authors: Collier, Scott
Citation: Collier, Scott; Voehler, Markus; Peng, Dungeng; Ohi, Ryomi; Gould, Kathleen; Reiter, Nicholas; Ohi, Melanie. "Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5" Biochemistry 53, 6439-6451 (2014).
Assembly members:
Cdc5-D3_(aa_155-214), polymer, 63 residues, 7471.6 Da.
Natural source: Common Name: E.coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15-b
Entity Sequences (FASTA):
Cdc5-D3_(aa_155-214): GSHMDEDEKEMLSEARARLA
NTQGKKAKRKDREKQLELTR
RLSHLQKRRELKAAGINIKL
FRR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 184 |
15N chemical shifts | 60 |
1H chemical shifts | 60 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Cdc5-D3 monomer | 1 |
Entities:
Entity 1, Cdc5-D3 monomer 63 residues - 7471.6 Da.
1 | GLY | SER | HIS | MET | ASP | GLU | ASP | GLU | LYS | GLU | ||||
2 | MET | LEU | SER | GLU | ALA | ARG | ALA | ARG | LEU | ALA | ||||
3 | ASN | THR | GLN | GLY | LYS | LYS | ALA | LYS | ARG | LYS | ||||
4 | ASP | ARG | GLU | LYS | GLN | LEU | GLU | LEU | THR | ARG | ||||
5 | ARG | LEU | SER | HIS | LEU | GLN | LYS | ARG | ARG | GLU | ||||
6 | LEU | LYS | ALA | ALA | GLY | ILE | ASN | ILE | LYS | LEU | ||||
7 | PHE | ARG | ARG |
Samples:
Cdc5-D3_(aa_155-214)_sample: Cdc5-D3 (aa 155-214), [U-99% 13C; U-99% 15N], 370 uM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 0.1 M; pH: 6; pressure: 1 atm; temperature: 293 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D HNCO | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D HNCA | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D HNCACB | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D HN(CO)CA | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D CBCA(CO)NH | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
3D HN(CA)CO | Cdc5-D3_(aa_155-214)_sample | anisotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts