BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34646

Title: Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles

Deposition date: 2021-07-08 Original release date: 2023-01-10

Authors: Wang, X.; Bouaziz, S.

Citation: Wang, X.; Bouaziz, S.. "Solution structures of HIV-1 and SIVmac p6 and their interaction with accessory proteins Vpr and Vpx in the presence of DPC micelles"  .

Assembly members:
entity_1, polymer, 52 residues, 5812.297 Da.

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:   Production method: recombinant technology   Host organism: Synthecium

Entity Sequences (FASTA):
entity_1: LQSRPEPTAPPEESFRFGEE TTTPSQKQEPIDKELYPLAS LRSLFGSDPSSQ

Data sets:
Data typeCount
15N chemical shifts6
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 52 residues - 5812.297 Da.

1   LEUGLNSERARGPROGLUPROTHRALAPRO
2   PROGLUGLUSERPHEARGPHEGLYGLUGLU
3   THRTHRTHRPROSERGLNLYSGLNGLUPRO
4   ILEASPLYSGLULEUTYRPROLEUALASER
5   LEUARGSERLEUPHEGLYSERASPPROSER
6   SERGLN

Samples:

sample_1: HIV-1 p6 1.0 mM

sample_conditions_1: ionic strength: 50 mM; pH: 4; pressure: 1 atm; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - processing

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, CCPN - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts