BMRB Entry 34680
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34680
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Title: Three-dimensional structure of the PGAM5 C12L mutant TMD PubMed: 35921890
Deposition date: 2021-11-17 Original release date: 2022-04-29
Authors: Silber, M.; Muhle-Goll, C.
Citation: Siebert, V.; Silber, M.; Heuten, E.; Muhle-Goll, C.; Lemberg, M.. "Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state" J. Biol. Chem. 298, 102321-102321 (2022).
Assembly members:
entity_1, polymer, 35 residues, 3314.816 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: AFRQALQLAALGLAGGSAAV
LFSAVAVGKPRAGGD
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 79 |
1H chemical shifts | 170 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 35 residues - 3314.816 Da.
1 | ALA | PHE | ARG | GLN | ALA | LEU | GLN | LEU | ALA | ALA | ||||
2 | LEU | GLY | LEU | ALA | GLY | GLY | SER | ALA | ALA | VAL | ||||
3 | LEU | PHE | SER | ALA | VAL | ALA | VAL | GLY | LYS | PRO | ||||
4 | ARG | ALA | GLY | GLY | ASP |
Samples:
sample_1: PGAM5 TMD 0.5 mM
sample_conditions_1: ionic strength: 1 Not defined; pH: 5.0; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS, Brunger A. T. et.al. - refinement
ARIA, Linge, O'Donoghue and Nilges - structure calculation
CcpNmr Analysis, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED. - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz