BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34681

Title: Three-dimensional structure of the PGAM5 WT TMD   PubMed: 35921890

Deposition date: 2021-11-17 Original release date: 2022-04-29

Authors: Silber, M.; Muhle-Goll, C.

Citation: Siebert, V.; Silber, M.; Heuten, E.; Muhle-Goll, C.; Lemberg, M.. "Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state"  J. Biol. Chem. 298, 102321-102321 (2022).

Assembly members:
entity_1, polymer, 35 residues, 3304.801 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: AFRQALQLAACGLAGGSAAV LFSAVAVGKPRAGGD

Data sets:
Data typeCount
13C chemical shifts89
1H chemical shifts193

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 35 residues - 3304.801 Da.

1   ALAPHEARGGLNALALEUGLNLEUALAALA
2   CYSGLYLEUALAGLYGLYSERALAALAVAL
3   LEUPHESERALAVALALAVALGLYLYSPRO
4   ARGALAGLYGLYASP

Samples:

sample_1: PGAM5 WT 0.5 mM

sample_conditions_1: ionic strength: 1 Not defined; pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker AVANCE III 600 MHz