BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4235

Title: NMR Solution Structure of [d(GCGAATTCGC)2]

Deposition date: 1998-09-29 Original release date: 1999-02-08

Authors: Aramini, James; Mujeeb, A.; Germann, Marcus

Citation: Aramini, James; Mujeeb, A.; Germann, Marcus. "NMR Solution Structures of [d(GCGAAT-3'-3'-alphaT-5'-5'-CGC)2] and its Unmodified Control"  Nucleic Acids Res. 15, 5644-5654 (1998).

Assembly members:
DNA decamer, polymer, 10 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
DNA decamer: GCGAATTCGC

Data sets:
Data typeCount
1H chemical shifts82
31P chemical shifts9

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1strand_A1
2strand_B1

Entities:

Entity 1, strand_A 10 residues - Formula weight is not available

1   DGDCDGDADADTDTDCDGDC

Samples:

sample_one: DNA decamer 2.6 mM; NaCl 50 mM; NaPhosphate 10 mM; EDTA 0.1 mM

sample_conditions_one: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_onenot availablesample_conditions_one
DQF-COSYsample_onenot availablesample_conditions_one
31P-1H correlationsample_onenot availablesample_conditions_one

Software:

XWINNMR - acquisition, processing, assignment

SPARKY v3.33 - cross-peak integration

NMR spectrometers:

  • Bruker AMX 600 MHz