BMRB Entry 4581
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4581
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Title: Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k
Deposition date: 2000-02-02 Original release date: 2000-12-18
Authors: Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.
Citation: Maler, L.; Blankenship, J.; Rance, M.; Chazin, W.. "Site-site Communication in the EF-hand Ca2+ Binding Protein Calbindin D9k" Nat. Struct. Biol. 7, 245-250 (2000).
Assembly members:
CALBINDIN D9K, polymer, 75 residues, Formula weight is not available
Natural source: Common Name: Bovine Taxonomy ID: 9913 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos taurus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
CALBINDIN D9K: KSPEELKGIFEKYAAKEGDP
NQLSKEELKLLLQTEFPSLL
KGMSTLDELFEELDKAGDGE
VSFEEFQVLVKKISQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 480 |
| 15N chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CALBINDIN D9K | 1 |
Entities:
Entity 1, CALBINDIN D9K 75 residues - Formula weight is not available
| 1 | LYS | SER | PRO | GLU | GLU | LEU | LYS | GLY | ILE | PHE | ||||
| 2 | GLU | LYS | TYR | ALA | ALA | LYS | GLU | GLY | ASP | PRO | ||||
| 3 | ASN | GLN | LEU | SER | LYS | GLU | GLU | LEU | LYS | LEU | ||||
| 4 | LEU | LEU | GLN | THR | GLU | PHE | PRO | SER | LEU | LEU | ||||
| 5 | LYS | GLY | MET | SER | THR | LEU | ASP | GLU | LEU | PHE | ||||
| 6 | GLU | GLU | LEU | ASP | LYS | ALA | GLY | ASP | GLY | GLU | ||||
| 7 | VAL | SER | PHE | GLU | GLU | PHE | GLN | VAL | LEU | VAL | ||||
| 8 | LYS | LYS | ILE | SER | GLN |
Samples:
sample_1: CALBINDIN D9K 4 mM
sample_2: CALBINDIN D9K, [U-15N], 4 mM
sample_cond_1: pH: 6.0; temperature: 300 K; ionic strength: 0 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 3D 15N-SEPARATED NOESY | not available | not available | sample_cond_1 |
| HSQC-J | not available | not available | sample_cond_1 |
Software:
FELIX v97.0 - resonance assignments, bookkeeping
DIANA v2.8 - structure solution
AMBER v4.1 - refinement
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 15594 16340 16758 247 325 326 327 |
| PDB | |
| GB | AAA30420 AAA72542 AAI18481 |
| PRF | 0707237A:PDB=3ICB |
| REF | NP_776682 XP_004021986 XP_005701114 XP_005888771 XP_005982038 |
| SP | P02633 |
| TPG | DAA12577 |
| AlphaFold | P02633 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts