BMRB Entry 4610
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4610
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Title: NMR Observation of A-tetrad in a DNA Quadruplex
Deposition date: 2000-05-31 Original release date: 2000-12-22
Authors: Patel, P.; Koti, A.; Hosur, R.
Citation: Patel, P.; Koti, A.; Hosur, R.. "NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad" Nucleic Acids Res. 27, 3836-3843 (1999).
Assembly members:
AGGGT, polymer, 5 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
AGGGT: AGGGT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 29 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 5'-D(*AP*GP*GP*GP*T)-3', strand 1a | 1 |
| 2 | 5'-D(*AP*GP*GP*GP*T)-3', strand 1b | 1 |
| 3 | 5'-D(*AP*GP*GP*GP*T)-3', strand 2a | 1 |
| 4 | 5'-D(*AP*GP*GP*GP*T)-3', strand 2b | 1 |
Entities:
Entity 1, 5'-D(*AP*GP*GP*GP*T)-3', strand 1a 5 residues - Formula weight is not available
| 1 | DA | DG | DG | DG | DT |
Samples:
sample_1: AGGGT1 – 2 mM; potassium phosphate 2 mM; EDTA 0.2 mM; KCl 100.0 mM; H2O 90%; D2O 10%
sample_2: AGGGT1 – 2 mM; potassium phosphate 2 mM; EDTA 0.2 mM; KCl 100.0 mM; D2O 100%
sample_cond_1: pH: 7.3; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| E-COSY | not available | not available | sample_cond_1 |
Software:
VNMR v6.1 - data collection
FELIX v230 and 97 - data analysis
xwinnmr v1.3 - data collection
DISCOVER v3.1 - structure solution
IRMA v2.3 - iterative matrix relaxation
NMR spectrometers:
- Bruker AMX 500 MHz