BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4850

Title: Glutaredoxin 3 from Escherichia coli in the fully oxidized form   PubMed: 11031118

Deposition date: 2000-10-06 Original release date: 2001-03-12

Authors: Nordstrand, K.; Sandstrom, A.; Aslund, F.; Holmgren, A.; Otting, G.; Berndt, K.

Citation: Nordstrand, K.; Sandstrom, A.; Aslund, F.; Holmgren, A.; Otting, G.; Berndt, K.. "NMR Structure of Oxidized Glutaredoxin 3 from Escherichia coli"  J. Mol. Biol. 303, 423-432 (2000).

Assembly members:
GLUTAREDOXIN 3, polymer, 82 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PET-3

Entity Sequences (FASTA):
GLUTAREDOXIN 3: ANVEIYTKETCPYCHRAKAL LSSKGVSFQELPIDGNAAKR EEMIKRSGRTTVPQIFIDAQ HIGGYDDLYALDARGGLDPL LK

Data sets:
Data typeCount
1H chemical shifts580
15N chemical shifts81

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GLUTAREDOXIN 31

Entities:

Entity 1, GLUTAREDOXIN 3 82 residues - Formula weight is not available

1   ALAASNVALGLUILETYRTHRLYSGLUTHR
2   CYSPROTYRCYSHISARGALALYSALALEU
3   LEUSERSERLYSGLYVALSERPHEGLNGLU
4   LEUPROILEASPGLYASNALAALALYSARG
5   GLUGLUMETILELYSARGSERGLYARGTHR
6   THRVALPROGLNILEPHEILEASPALAGLN
7   HISILEGLYGLYTYRASPASPLEUTYRALA
8   LEUASPALAARGGLYGLYLEUASPPROLEU
9   LEULYS

Samples:

sample_1: GLUTAREDOXIN 3 mM

sample_2: GLUTAREDOXIN 3, [U-15N], mM

sample_cond_1: pH: 5.5; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions

Software:

PROSA v3.6 - processing

XEASY v1.4 - data analysis

DYANA v1.5 - refinement

OPAL v2.6 - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4225
PDB
DBJ BAB37911 BAE77682 BAG79416 BAI28143 BAI33266
EMBL CAP78066 CAQ33936 CAR00580 CAR05236 CAR10284
GB AAB18587 AAC76634 AAG58757 AAN45096 AAN82869
REF NP_290193 NP_312515 NP_418067 NP_709389 NP_756295
SP P0AC62 P0AC63 P0AC64
AlphaFold P0AC64 P0AC63 P0AC62

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts