BMRB Entry 4996
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4996
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Title: NMR-Based Structure of the Conserved Protein MTH865 from the Archea Methanobacterium thermoautotrophicum
Deposition date: 2001-04-20 Original release date: 2001-10-08
Authors: Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence
Citation: Lee, Gregory; Edwards, Aled; Arrowsmith, Cheryl; McIntosh, Lawrence. "Letter to the Editor: NMR-Based Structure of the Conserved Protein MTH865 from the Archaeon Methanobacterium thermoautotrophicum" J. Biomol. NMR 21, 63-66 (2001).
Assembly members:
Methanobacterium thermoautotrophicum open reading frame 865, polymer, 84 residues, 8675 Da.
Natural source: Common Name: Methanothermobacter thermautotrophicus Taxonomy ID: 145262 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Methanothermobacter thermautotrophicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
Methanobacterium thermoautotrophicum open reading frame 865: GSHMKMGVKEDIRGQIIGAL
AGADFPINSPEELMAALPNG
PDTTCKSGDVELKASDAGQV
LTADDFPFKSAEEVADTIVN
KAGL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 540 |
| 13C chemical shifts | 324 |
| 15N chemical shifts | 81 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MTH865 | 1 |
Entities:
Entity 1, MTH865 84 residues - 8675 Da.
| 1 | GLY | SER | HIS | MET | LYS | MET | GLY | VAL | LYS | GLU | ||||
| 2 | ASP | ILE | ARG | GLY | GLN | ILE | ILE | GLY | ALA | LEU | ||||
| 3 | ALA | GLY | ALA | ASP | PHE | PRO | ILE | ASN | SER | PRO | ||||
| 4 | GLU | GLU | LEU | MET | ALA | ALA | LEU | PRO | ASN | GLY | ||||
| 5 | PRO | ASP | THR | THR | CYS | LYS | SER | GLY | ASP | VAL | ||||
| 6 | GLU | LEU | LYS | ALA | SER | ASP | ALA | GLY | GLN | VAL | ||||
| 7 | LEU | THR | ALA | ASP | ASP | PHE | PRO | PHE | LYS | SER | ||||
| 8 | ALA | GLU | GLU | VAL | ALA | ASP | THR | ILE | VAL | ASN | ||||
| 9 | LYS | ALA | GLY | LEU |
Samples:
15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 15N], 0.5 mM
13C_15N-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-100% 13C; U-100% 15N], 0.5 mM
10%_13C-labeled_sample: Methanobacterium thermoautotrophicum open reading frame 865, [U-10% 13C; U-100% 15N], 0.5 mM
condition_1: pH: 6.51; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 500MHz Unity housed at Univ. of British Columbia | not available | not available | condition_1 |
| 600MHz INOVA housed at Univ. of Toronto | not available | not available | condition_1 |
| 800MHz INOVA housed at Univ. of Alberta | not available | not available | condition_1 |
Software:
Felix v2000 - raw data processing, peak picking, peak assignments
NMR spectrometers:
- Varian UNITY 500 MHz
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAM70032 |
| EMBL | CAA48863 CAA48865 |
| GB | AAB85363 |
| REF | NP_276002 WP_010876498 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts