BMRB Entry 50192

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50192

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Title:
Solid state NMR assignments for the patient FOR005 l-III immunoglobulin light chain variable domain amyloid fibrils
Deposition date:
2020-02-07
Original release date:
2020-09-19
Authors:
Pradhan, Tejaswini; Annamalai, Karthikeyan; Sarkar, Riddhiman; Hegenbart, Ute; Schonland, Stefan; Fandrich, Marcus; Reif, Bernd
Citation:

Citation: Pradhan, Tejaswini; Annamalai, Karthikeyan; Sarkar, Riddhiman; Hegenbart, Ute; Schonland, Stefan; Fandrich, Marcus; Reif, Bernd. "Solid state NMR assignments of a human l-III immunoglobulin light chain amyloid fibrils"  Biomol. NMR Assign. 15, 9-16 (2021).
PubMed: 32946005

Assembly members:

Assembly members:
entity_1, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28(b+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSSELTQDPAVSVALGQTVR ITCQGDSLRSYSASWYQQKP GQAPVLVIFRKSNRPSGIPD RFSGSSSGNTASLTITGAQA EDEADYYCNSRDSSANHQVF GGGTKLTVLG

Data sets:
Data typeCount
13C chemical shifts210
15N chemical shifts51

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S3706_VL1

Entities:

Entity 1, S3706_VL 110 residues - Formula weight is not available

1   GLYSERSERGLULEUTHRGLNASPPROALA
2   VALSERVALALALEUGLYGLNTHRVALARG
3   ILETHRCYSGLNGLYASPSERLEUARGSER
4   TYRSERALASERTRPTYRGLNGLNLYSPRO
5   GLYGLNALAPROVALLEUVALILEPHEARG
6   LYSSERASNARGPROSERGLYILEPROASP
7   ARGPHESERGLYSERSERSERGLYASNTHR
8   ALASERLEUTHRILETHRGLYALAGLNALA
9   GLUASPGLUALAASPTYRTYRCYSASNSER
10   ARGASPSERSERALAASNHISGLNVALPHE
11   GLYGLYGLYTHRLYSLEUTHRVALLEUGLY

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 50 uM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D PDSDsample_1fibrilssample_conditions_1
2D DARRsample_1fibrilssample_conditions_1
2D NCAsample_1fibrilssample_conditions_1
2D NCACXsample_1fibrilssample_conditions_1
2D NCOCXsample_1fibrilssample_conditions_1
3D NCACXsample_1fibrilssample_conditions_1
3D NCOCXsample_1fibrilssample_conditions_1
3D CONCAsample_1fibrilssample_conditions_1
3D CANCOsample_1fibrilssample_conditions_1

Software:

CcpNMR vV2, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz