BMRB Entry 51023
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51023
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: FLN5 A3A3 PubMed: 34650236
Deposition date: 2021-07-22 Original release date: 2021-08-23
Authors: Cassaignau, Anais; Wlodarski, Tomasz; Chan, Sammy; Woodburn, Lauren; Vujkovic Bukvin, Ivana; Streit, Julian; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John
Citation: Cassaignau, Anais; Wlodarski, Tomasz; Chan, Sammy; Woodburn, Lauren; Vujkovic Bukvin, Ivana; Streit, Julian; Cabrita, Lisa; Waudby, Christopher; Christodoulou, John. "Interactions between nascent proteins and the ribosome surface inhibit co-translational folding" Nat. Chem. 13, 1214-1220 (2021).
Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available
Natural source: Common Name: Dictyostelium discoideum Taxonomy ID: 44689 Superkingdom: Eukaryota Kingdom: not available Genus/species: Dictyostelium discoideum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pLDC
Entity Sequences (FASTA):
entity_1: MHHHHHHASKPAPSAEHSYA
EGEGNVKVADNAPAEATIAA
VDTKGVARTDGGDPFEVAIN
GPDGLVVDAKVTDNNDGTAG
VVADAPVEGNANVNVTLRGN
PIKNMPIDVKCIEGPIDVKV
IEGPIDVKVIEGPIDVKVIE
GPIDVKCIEG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FLN5 A3A3 | 1 |
Entities:
Entity 1, FLN5 A3A3 150 residues - Formula weight is not available
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | ALA | SER | LYS | |
| 2 | PRO | ALA | PRO | SER | ALA | GLU | HIS | SER | TYR | ALA | |
| 3 | GLU | GLY | GLU | GLY | ASN | VAL | LYS | VAL | ALA | ASP | |
| 4 | ASN | ALA | PRO | ALA | GLU | ALA | THR | ILE | ALA | ALA | |
| 5 | VAL | ASP | THR | LYS | GLY | VAL | ALA | ARG | THR | ASP | |
| 6 | GLY | GLY | ASP | PRO | PHE | GLU | VAL | ALA | ILE | ASN | |
| 7 | GLY | PRO | ASP | GLY | LEU | VAL | VAL | ASP | ALA | LYS | |
| 8 | VAL | THR | ASP | ASN | ASN | ASP | GLY | THR | ALA | GLY | |
| 9 | VAL | VAL | ALA | ASP | ALA | PRO | VAL | GLU | GLY | ASN | |
| 10 | ALA | ASN | VAL | ASN | VAL | THR | LEU | ARG | GLY | ASN | |
| 11 | PRO | ILE | LYS | ASN | MET | PRO | ILE | ASP | VAL | LYS | |
| 12 | CYS | ILE | GLU | GLY | PRO | ILE | ASP | VAL | LYS | VAL | |
| 13 | ILE | GLU | GLY | PRO | ILE | ASP | VAL | LYS | VAL | ILE | |
| 14 | GLU | GLY | PRO | ILE | ASP | VAL | LYS | VAL | ILE | GLU | |
| 15 | GLY | PRO | ILE | ASP | VAL | LYS | CYS | ILE | GLU | GLY |
Samples:
sample_1: FLN5 A3A3, [U-100% 15N], 150 uM; HEPES 10 mM; NH4Cl 30 mM; MgCl2 12 mM; EDTA 5 mM; DSS 0.001%; D2O 10%
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HMQC | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts