BMRB Entry 51373
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51373
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Title: 15N-1H chemical shifts for the murine Syk tandem SH2 domains flexible linker construct [tSH2-FX, Ser 8 to Gln 264 with interdomain residues 119-162 replaced by a 20-amino-acid flexible linker (GGS)3GS(GGS)3] complexed with ITP peptide PubMed: 26468009
Deposition date: 2022-03-18 Original release date: 2022-04-18
Authors: Feng, Chao; Post, Carol
Citation: Feng, Chao; Post, Carol Beth. "Insights into the allosteric regulation of Syk association with receptor ITAM, a multi-state equilibrium." Phys. Chem. Chem. Phys. 18, 5807-5818 (2016).
Assembly members:
entity_1, polymer, 233 residues, Formula weight is not available
entity_2, polymer, 22 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-30a(+)
Entity Sequences (FASTA):
entity_1: SANHLTYFFGNITREEAEDY
LVQGGMTDGLYLLRQSRNYL
GGFALSVAHNRKAHHYTIER
ELNGTYAISGGRAHASPADL
CHYHSQEPDGLICLLKKPFN
RPPGVQPKTGPGGSGGSGGS
GSGGSGGSGGSEKMPWFHGN
ISRDESEQTVLIGSKTNGKF
LIRARDNSGSYALCLLHEGK
VLHYRIDRDKTGKLSIPEGK
KFDTLWQLVEHYSYKPDGLL
RVLTVPCQKIGTQ
entity_2: XPDXEPIRKGQRDLXSGLNQ
RX
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 124 |
| 1H chemical shifts | 124 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | murine Syk tandem SH2 domains flexible linker construct | 1 |
| 2 | ITP peptide | 2 |
Entities:
Entity 1, murine Syk tandem SH2 domains flexible linker construct 233 residues - Formula weight is not available
| 1 | SER | ALA | ASN | HIS | LEU | THR | TYR | PHE | PHE | GLY | ||||
| 2 | ASN | ILE | THR | ARG | GLU | GLU | ALA | GLU | ASP | TYR | ||||
| 3 | LEU | VAL | GLN | GLY | GLY | MET | THR | ASP | GLY | LEU | ||||
| 4 | TYR | LEU | LEU | ARG | GLN | SER | ARG | ASN | TYR | LEU | ||||
| 5 | GLY | GLY | PHE | ALA | LEU | SER | VAL | ALA | HIS | ASN | ||||
| 6 | ARG | LYS | ALA | HIS | HIS | TYR | THR | ILE | GLU | ARG | ||||
| 7 | GLU | LEU | ASN | GLY | THR | TYR | ALA | ILE | SER | GLY | ||||
| 8 | GLY | ARG | ALA | HIS | ALA | SER | PRO | ALA | ASP | LEU | ||||
| 9 | CYS | HIS | TYR | HIS | SER | GLN | GLU | PRO | ASP | GLY | ||||
| 10 | LEU | ILE | CYS | LEU | LEU | LYS | LYS | PRO | PHE | ASN | ||||
| 11 | ARG | PRO | PRO | GLY | VAL | GLN | PRO | LYS | THR | GLY | ||||
| 12 | PRO | GLY | GLY | SER | GLY | GLY | SER | GLY | GLY | SER | ||||
| 13 | GLY | SER | GLY | GLY | SER | GLY | GLY | SER | GLY | GLY | ||||
| 14 | SER | GLU | LYS | MET | PRO | TRP | PHE | HIS | GLY | ASN | ||||
| 15 | ILE | SER | ARG | ASP | GLU | SER | GLU | GLN | THR | VAL | ||||
| 16 | LEU | ILE | GLY | SER | LYS | THR | ASN | GLY | LYS | PHE | ||||
| 17 | LEU | ILE | ARG | ALA | ARG | ASP | ASN | SER | GLY | SER | ||||
| 18 | TYR | ALA | LEU | CYS | LEU | LEU | HIS | GLU | GLY | LYS | ||||
| 19 | VAL | LEU | HIS | TYR | ARG | ILE | ASP | ARG | ASP | LYS | ||||
| 20 | THR | GLY | LYS | LEU | SER | ILE | PRO | GLU | GLY | LYS | ||||
| 21 | LYS | PHE | ASP | THR | LEU | TRP | GLN | LEU | VAL | GLU | ||||
| 22 | HIS | TYR | SER | TYR | LYS | PRO | ASP | GLY | LEU | LEU | ||||
| 23 | ARG | VAL | LEU | THR | VAL | PRO | CYS | GLN | LYS | ILE | ||||
| 24 | GLY | THR | GLN |
Entity 2, ITP peptide 22 residues - Formula weight is not available
| 1 | ACE | PRO | ASP | PTR | GLU | PRO | ILE | ARG | LYS | GLY | ||||
| 2 | GLN | ARG | ASP | LEU | PTR | SER | GLY | LEU | ASN | GLN | ||||
| 3 | ARG | NH2 |
Samples:
sample_1: murine Syk tandem SH2 domains flexible linker construct, [U-99% 15N], 0.3 mM; sodium phosphate 50 mM; DTT 5 mM; sodium azide 0.02%; ITP peptide 1.8 mM
sample_conditions_1: ionic strength: 0.13 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts