BMRB Entry 51494
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51494
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TDP-43 low complexity domain with methionine sulfoxide modifications PubMed: 36709343
Deposition date: 2022-06-16 Original release date: 2023-01-30
Authors: Carrasco, Jaime; Anton, Rosa; Pantoja-Uceda, David; Laurents, Douglas; Oroz, Javier
Citation: Carrasco, Jaime; Anton, Rosa; Valbuena, Alejandro; Pantoja-Uceda, David; Mukhi, Mayur; Hervas, Ruben; Laurents, Douglas; Gasset, Maria; Oroz, Javier. "Metamorphism in TDP-43 prion-like domain determines chaperone recognition" Nat. Commun. 14, 466-466 (2023).
Assembly members:
entity_1, polymer, 141 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: GRFGGNPGGFGNQGGFGNSR
GGGAGLGNNQGSNXGGGXNF
GAFSINPAXXAAAQAALQSS
WGXXGXLASQQNQSGPSGNN
QNQGNXQREPNQAFGSGNNS
YSGSNSGAAIGWGSASNAGS
GSGFNGGFGSSXDSKSSGWG
X
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 351 |
| 15N chemical shifts | 136 |
| 1H chemical shifts | 457 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TDP-43 PLD | 1 |
Entities:
Entity 1, TDP-43 PLD 141 residues - Formula weight is not available
| 1 | GLY | ARG | PHE | GLY | GLY | ASN | PRO | GLY | GLY | PHE | ||||
| 2 | GLY | ASN | GLN | GLY | GLY | PHE | GLY | ASN | SER | ARG | ||||
| 3 | GLY | GLY | GLY | ALA | GLY | LEU | GLY | ASN | ASN | GLN | ||||
| 4 | GLY | SER | ASN | MHO | GLY | GLY | GLY | MHO | ASN | PHE | ||||
| 5 | GLY | ALA | PHE | SER | ILE | ASN | PRO | ALA | MHO | MHO | ||||
| 6 | ALA | ALA | ALA | GLN | ALA | ALA | LEU | GLN | SER | SER | ||||
| 7 | TRP | GLY | MHO | MHO | GLY | MHO | LEU | ALA | SER | GLN | ||||
| 8 | GLN | ASN | GLN | SER | GLY | PRO | SER | GLY | ASN | ASN | ||||
| 9 | GLN | ASN | GLN | GLY | ASN | MHO | GLN | ARG | GLU | PRO | ||||
| 10 | ASN | GLN | ALA | PHE | GLY | SER | GLY | ASN | ASN | SER | ||||
| 11 | TYR | SER | GLY | SER | ASN | SER | GLY | ALA | ALA | ILE | ||||
| 12 | GLY | TRP | GLY | SER | ALA | SER | ASN | ALA | GLY | SER | ||||
| 13 | GLY | SER | GLY | PHE | ASN | GLY | GLY | PHE | GLY | SER | ||||
| 14 | SER | MHO | ASP | SER | LYS | SER | SER | GLY | TRP | GLY | ||||
| 15 | MHO |
Samples:
sample_1: TDP-43 PLD, [U-98% 13C; U-98% 15N], 0.3 mM; HEPES 20 mM; KCL 10 mM; MgCl2 5 mM; ATP 1 mM; DTT 1 mM; PMSF 0.2 mM; sodium azide 0.03%; DSS 0.5 mM
sample_conditions_1: ionic strength: 0.01 M; pH: 6.8; pressure: 1 atm; temperature: 288 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY v3.190 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts