BMRB Entry 51770
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51770
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Title: Chemical shifts of UBQLN1 514-589, F547Y at 600 micromolar PubMed: 37022617
Deposition date: 2023-01-09 Original release date: 2023-01-30
Authors: Buel, Gwen; Chen, Xiang; Walters, Kylie
Citation: Buel, Gwen; Chen, Xiang; Kayode, Olumide; Cruz, Anthony; Walters, Kylie. "1H, 15N, 13C backbone and Cb resonance assignments for UBQLN1 UBA and UBAA domains" Biomol. NMR Assignments 17, 101-106 (2023).
Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6P
Entity Sequences (FASTA):
entity_1: TAGTTEPGHQQFIQQMLQAL
AGVNPQLQNPEVRYQQQLEQ
LSAMGFLNREANLQALIATG
GDINAAIERLLGSQPS
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 107 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UBQLN1 514-589, F547Y 600uM | 1 |
Entities:
Entity 1, UBQLN1 514-589, F547Y 600uM 76 residues - Formula weight is not available
| 1 | THR | ALA | GLY | THR | THR | GLU | PRO | GLY | HIS | GLN | ||||
| 2 | GLN | PHE | ILE | GLN | GLN | MET | LEU | GLN | ALA | LEU | ||||
| 3 | ALA | GLY | VAL | ASN | PRO | GLN | LEU | GLN | ASN | PRO | ||||
| 4 | GLU | VAL | ARG | TYR | GLN | GLN | GLN | LEU | GLU | GLN | ||||
| 5 | LEU | SER | ALA | MET | GLY | PHE | LEU | ASN | ARG | GLU | ||||
| 6 | ALA | ASN | LEU | GLN | ALA | LEU | ILE | ALA | THR | GLY | ||||
| 7 | GLY | ASP | ILE | ASN | ALA | ALA | ILE | GLU | ARG | LEU | ||||
| 8 | LEU | GLY | SER | GLN | PRO | SER |
Samples:
sample_1: UBQLN1 514-589, F547Y at 600uM, [U-100% 15N], 600 uM; MOPS 10 mM; NaCl 50 mM; DTT 5 mM; zinc sulfate 10 uM; pefabloc 1 mM; NaN3 0.1%
sample_conditions_1: ionic strength: 60 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 15N-separated NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
XEASY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts