BMRB Entry 51793
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51793
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Title: FapC Full-Length PubMed: 37162737
Deposition date: 2023-01-22 Original release date: 2023-01-27
Authors: Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; akbey, umit
Citation: Byeon, Chang-Hyeock; Wang, Pang; Byeon, In-Ja; Akbey, Umit. "Solution-state NMR assignment and secondary structure propensity of the full length and minimalistic-truncated prefibrillar monomeric form of biofilm forming functional amyloid FapC from Pseudomonas aeruginosa" Biomol. NMR Assign. 17, 159-165 (2023).
Assembly members:
entity_1, polymer, 235 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MGPAEKWKPTPAPTGTVAAA
VTDTQVSKDNKFDDTKTLNN
AGANGSLSNSKGNLGANIAA
GSGNQQDNAAAITSSAGDAA
TVFAVADIYQESKDNKFTNK
GTQNNALLNNSANNSSGNVG
VNVAAGQGNQQKNNLAIVTA
DGKNVAAASNTEQVSLDNHF
LNEASSKHSYKPQYVVNNAG
LLNSANNASGNIGVNVAAGA
GNQQSNTLTLGSGCTVCAAG
TGSKLAFLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 417 |
15N chemical shifts | 198 |
1H chemical shifts | 764 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | FapC Full-Length | 1 |
Entities:
Entity 1, FapC Full-Length 235 residues - Formula weight is not available
Residue 1 is a nonnative Methionine. The last 8 residues are nonnative and due to the His-tag. The original aminoacids and the numbering in the FapC FapC is as: G25,P26,A27,...
1 | MET | GLY | PRO | ALA | GLU | LYS | TRP | LYS | PRO | THR | ||||
2 | PRO | ALA | PRO | THR | GLY | THR | VAL | ALA | ALA | ALA | ||||
3 | VAL | THR | ASP | THR | GLN | VAL | SER | LYS | ASP | ASN | ||||
4 | LYS | PHE | ASP | ASP | THR | LYS | THR | LEU | ASN | ASN | ||||
5 | ALA | GLY | ALA | ASN | GLY | SER | LEU | SER | ASN | SER | ||||
6 | LYS | GLY | ASN | LEU | GLY | ALA | ASN | ILE | ALA | ALA | ||||
7 | GLY | SER | GLY | ASN | GLN | GLN | ASP | ASN | ALA | ALA | ||||
8 | ALA | ILE | THR | SER | SER | ALA | GLY | ASP | ALA | ALA | ||||
9 | THR | VAL | PHE | ALA | VAL | ALA | ASP | ILE | TYR | GLN | ||||
10 | GLU | SER | LYS | ASP | ASN | LYS | PHE | THR | ASN | LYS | ||||
11 | GLY | THR | GLN | ASN | ASN | ALA | LEU | LEU | ASN | ASN | ||||
12 | SER | ALA | ASN | ASN | SER | SER | GLY | ASN | VAL | GLY | ||||
13 | VAL | ASN | VAL | ALA | ALA | GLY | GLN | GLY | ASN | GLN | ||||
14 | GLN | LYS | ASN | ASN | LEU | ALA | ILE | VAL | THR | ALA | ||||
15 | ASP | GLY | LYS | ASN | VAL | ALA | ALA | ALA | SER | ASN | ||||
16 | THR | GLU | GLN | VAL | SER | LEU | ASP | ASN | HIS | PHE | ||||
17 | LEU | ASN | GLU | ALA | SER | SER | LYS | HIS | SER | TYR | ||||
18 | LYS | PRO | GLN | TYR | VAL | VAL | ASN | ASN | ALA | GLY | ||||
19 | LEU | LEU | ASN | SER | ALA | ASN | ASN | ALA | SER | GLY | ||||
20 | ASN | ILE | GLY | VAL | ASN | VAL | ALA | ALA | GLY | ALA | ||||
21 | GLY | ASN | GLN | GLN | SER | ASN | THR | LEU | THR | LEU | ||||
22 | GLY | SER | GLY | CYS | THR | VAL | CYS | ALA | ALA | GLY | ||||
23 | THR | GLY | SER | LYS | LEU | ALA | PHE | LEU | GLU | HIS | ||||
24 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: FapC Full-Lentgh, [U-13C; U-15N], 0.3 mM; DSS, [U-99% 2H], 1 mM; D2O, [U-99% 2H], 10%; DTT 30 mM; sodium phosphate 50 mM; sodium azide 0.02%
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 274 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v3.1 - chemical shift assignment
TOPSPIN v3 - collection
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker Avance 700 MHz
- Bruker Avance 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts