BMRB Entry 52354

Name: Backbone assignment of Hrp48 RRM2
Published: 2024-12-26

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52354

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone assignment of Hrp48 RRM2

Deposition date:

2024-03-14

Original release date:

2024-12-26

Authors:

Lomoschitz, Andrea; Meyer, Julia; Schweimer, Kristian; Hennig, Janosch

Citation:

Citation: Lomoschitz, Andrea; Meyer, Julia; Guitart, Tanit; Krepl, Miroslav; Lapouge, Karine; Hayn, Clara; Schweimer, Kristian; Simon, Bernd; Sponer, Jiri; Gebauer, Fatima; Hennig, Janosch. "The Drosophila RNA binding protein Hrp48 binds a specific RNA sequence of the msl-2 mRNA 3' UTR to regulate translation" Biophys. Chem. 316, 107346-107346 (2025).
PubMed: 39504588

Assembly members:

Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GAKPKKGGGYKVFLGGLPSN VTETDLRTFFNRYGKVTEVV IMYDQEKKKSRGFGFLSFEE ESSVEHVTNERYINLNGKQV EIKKAEP

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	204
15N chemical shifts	76
1H chemical shifts	76

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hrp48 RRM2	1

Entities:

Entity 1, Hrp48 RRM2 87 residues - Formula weight is not available

N-terminal GA from expression tag

1

GLY

ALA

LYS

PRO

LYS

GLY

TYR

2

LYS

VAL

PHE

LEU

GLY

LEU

PRO

SER

ASN

3

VAL

THR

GLU

THR

ASP

LEU

ARG

THR

PHE

4

ASN

ARG

TYR

GLY

LYS

VAL

THR

GLU

VAL

5

ILE

MET

TYR

ASP

GLN

GLU

LYS

SER

6

ARG

GLY

PHE

GLY

PHE

LEU

SER

PHE

GLU

7

GLU

SER

VAL

GLU

HIS

VAL

THR

ASN

GLU

8

ARG

TYR

ILE

ASN

LEU

ASN

GLY

LYS

GLN

VAL

9

GLU

ILE

LYS

ALA

GLU

PRO

Samples:

sample_1: Hrp48 RRM2, [U-95% 13C; U-95% 15N], 220 uM; MES 20 mM; sodium chloride 200 mM; D2O, [U-2H], 5%

sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks