BMRB Entry 5236
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5236
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Title: 1H and 15N Chemical Shift Assignments for the tandem inactivation domain of Kv1.4 (RCK4(1-75)) PubMed: 12590144
Deposition date: 2001-12-18 Original release date: 2003-04-30
Authors: Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Jonas, Peter; Bentrop, Detlef; Fakler, Bernd
Citation: Wissmann, Ralph; Bildl, Wolfgang; Oliver, Dominik; Beyermann, M.; Kalbitzer, H.; Bentrop, Detlef; Fakler, Bernd. "Solution Structure and Function of the "Tandem Inactivation Domain" of the Neuronal A-type Potassium Channel Kv1.4." J. Biol. Chem. 278, 16142-16150 (2003).
Assembly members:
RCK4, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET41a
Entity Sequences (FASTA):
RCK4: GSTMEVAMVSAESSGCNSHM
PYGYAAQARARERERLAHSR
AAAAAAVAAATAAVEGTGGS
GGGPHHHHQTRGAYSSHD
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 397 |
| 15N chemical shifts | 76 |
| coupling constants | 62 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RCK4(1-75) | 1 |
Entities:
Entity 1, RCK4(1-75) 78 residues - Formula weight is not available
| 1 | GLY | SER | THR | MET | GLU | VAL | ALA | MET | VAL | SER | ||||
| 2 | ALA | GLU | SER | SER | GLY | CYS | ASN | SER | HIS | MET | ||||
| 3 | PRO | TYR | GLY | TYR | ALA | ALA | GLN | ALA | ARG | ALA | ||||
| 4 | ARG | GLU | ARG | GLU | ARG | LEU | ALA | HIS | SER | ARG | ||||
| 5 | ALA | ALA | ALA | ALA | ALA | ALA | VAL | ALA | ALA | ALA | ||||
| 6 | THR | ALA | ALA | VAL | GLU | GLY | THR | GLY | GLY | SER | ||||
| 7 | GLY | GLY | GLY | PRO | HIS | HIS | HIS | HIS | GLN | THR | ||||
| 8 | ARG | GLY | ALA | TYR | SER | SER | HIS | ASP |
Samples:
sample_1: RCK4, [U-98% 15N], 0.8 – 1.0 mM; sodium phosphate 50 mM; DTT 10 mM; pefablock SC 1 mM
Ex-cond_1: pH: 4.4; temperature: 283 K; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| HNHA | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| BMRB | 19426 |
| PDB | |
| DBJ | BAC29309 |
| EMBL | CAA34133 |
| GB | AAA41469 AAB60668 AAI09015 EDL27774 EDL79747 |
| REF | NP_037103 NP_067250 XP_003497608 XP_005064870 XP_005364106 |
| SP | P15385 Q61423 |
| AlphaFold | Q61423 P15385 |
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