BMRB Entry 52364
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52364
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Title: The methyl assignment of E coli Hsp90 N domain PubMed: 38890550
Deposition date: 2024-03-26 Original release date: 2024-07-22
Authors: Qu, Xiaozhan
Citation: Qu, Xiaozhan; Zhao, Shuo; Wan, Chanjuan; Zhu, Lei; Ji, Tuo; Rossi, Paolo; Wang, Junfeng; Kalodimos, Charalampos; Wang, Chao; Xu, Weiya; Huang, Chengdong. "Structural basis for the dynamic chaperoning of disordered clients by Hsp90" Nat. Struct. Mol. Biol. ., .-. (2024).
Assembly members:
entity_1, polymer, 215 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-16a
Entity Sequences (FASTA):
entity_1: MKGQETRGFQSEVKQLLHLM
IHSLYSNKEIFLRELISNAS
DAADKLRFRALSNPDLYEGD
GELRVRVSFDKDKRTLTISD
NGVGMTRDEVIDHLGTIAKS
GTKSFLESLGSDQAKDSQLI
GQFGVGFYSAFIVADKVTVR
TRAAGEKPENGVFWESAGEG
EYTVADITKEDRGTEITLHL
REGEDEFLDDWRVRSIISKY
SDHIALPVEIEKREE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 101 |
| 1H chemical shifts | 306 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The NTD of E coli Hsp90 | 1 |
Entities:
Entity 1, The NTD of E coli Hsp90 215 residues - Formula weight is not available
| 1 | MET | LYS | GLY | GLN | GLU | THR | ARG | GLY | PHE | GLN | ||||
| 2 | SER | GLU | VAL | LYS | GLN | LEU | LEU | HIS | LEU | MET | ||||
| 3 | ILE | HIS | SER | LEU | TYR | SER | ASN | LYS | GLU | ILE | ||||
| 4 | PHE | LEU | ARG | GLU | LEU | ILE | SER | ASN | ALA | SER | ||||
| 5 | ASP | ALA | ALA | ASP | LYS | LEU | ARG | PHE | ARG | ALA | ||||
| 6 | LEU | SER | ASN | PRO | ASP | LEU | TYR | GLU | GLY | ASP | ||||
| 7 | GLY | GLU | LEU | ARG | VAL | ARG | VAL | SER | PHE | ASP | ||||
| 8 | LYS | ASP | LYS | ARG | THR | LEU | THR | ILE | SER | ASP | ||||
| 9 | ASN | GLY | VAL | GLY | MET | THR | ARG | ASP | GLU | VAL | ||||
| 10 | ILE | ASP | HIS | LEU | GLY | THR | ILE | ALA | LYS | SER | ||||
| 11 | GLY | THR | LYS | SER | PHE | LEU | GLU | SER | LEU | GLY | ||||
| 12 | SER | ASP | GLN | ALA | LYS | ASP | SER | GLN | LEU | ILE | ||||
| 13 | GLY | GLN | PHE | GLY | VAL | GLY | PHE | TYR | SER | ALA | ||||
| 14 | PHE | ILE | VAL | ALA | ASP | LYS | VAL | THR | VAL | ARG | ||||
| 15 | THR | ARG | ALA | ALA | GLY | GLU | LYS | PRO | GLU | ASN | ||||
| 16 | GLY | VAL | PHE | TRP | GLU | SER | ALA | GLY | GLU | GLY | ||||
| 17 | GLU | TYR | THR | VAL | ALA | ASP | ILE | THR | LYS | GLU | ||||
| 18 | ASP | ARG | GLY | THR | GLU | ILE | THR | LEU | HIS | LEU | ||||
| 19 | ARG | GLU | GLY | GLU | ASP | GLU | PHE | LEU | ASP | ASP | ||||
| 20 | TRP | ARG | VAL | ARG | SER | ILE | ILE | SER | LYS | TYR | ||||
| 21 | SER | ASP | HIS | ILE | ALA | LEU | PRO | VAL | GLU | ILE | ||||
| 22 | GLU | LYS | ARG | GLU | GLU |
Samples:
sample_1: The NTD of E coli Hsp90, [U-2H, 15N; Ala- 13CH3; Met- 13CH3; Ile-d1- 13CH3; Leu, Val- 13CH3/ 13CH3; Thr- 13CH3], 600 uM; Phosphate 50 mM; NaCl 100 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMRViewJ - chemical shift assignment
NMR spectrometers:
- AVANCE III AVANCE III 600 MHz