BMRB Entry 52366
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52366
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Title: The methyl assignment of E coli Hsp90 C domain PubMed: 38890550
Deposition date: 2024-03-26 Original release date: 2024-07-22
Authors: Qu, Xiaozhan
Citation: Qu, Xiaozhan; Zhao, Shuo; Wan, Chanjuan; Zhu, Lei; Ji, Tuo; Rossi, Paolo; Wang, Junfeng; Kalodimos, Charalampos; Wang, Chao; Xu, Weiya; Huang, Chengdong. "Structural basis for the dynamic chaperoning of disordered clients by Hsp90" Nat. Struct. Mol. Biol. ., .-. (2024).
Assembly members:
entity_1, polymer, 114 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16a
Entity Sequences (FASTA):
entity_1: FIDRVKALLGERVKDVRLTH
RLTDTPAIVSTDADEMSTQM
AKLFAAAGQKVPEVKYIFEL
NPDHVLVKRAADTEDEAKFS
EWVELLLDQALLAERGTLED
PNLFIRRMNQLLVS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 74 |
| 1H chemical shifts | 222 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | The CTD of E coli Hsp90 | 1 |
Entities:
Entity 1, The CTD of E coli Hsp90 114 residues - Formula weight is not available
| 1 | PHE | ILE | ASP | ARG | VAL | LYS | ALA | LEU | LEU | GLY | ||||
| 2 | GLU | ARG | VAL | LYS | ASP | VAL | ARG | LEU | THR | HIS | ||||
| 3 | ARG | LEU | THR | ASP | THR | PRO | ALA | ILE | VAL | SER | ||||
| 4 | THR | ASP | ALA | ASP | GLU | MET | SER | THR | GLN | MET | ||||
| 5 | ALA | LYS | LEU | PHE | ALA | ALA | ALA | GLY | GLN | LYS | ||||
| 6 | VAL | PRO | GLU | VAL | LYS | TYR | ILE | PHE | GLU | LEU | ||||
| 7 | ASN | PRO | ASP | HIS | VAL | LEU | VAL | LYS | ARG | ALA | ||||
| 8 | ALA | ASP | THR | GLU | ASP | GLU | ALA | LYS | PHE | SER | ||||
| 9 | GLU | TRP | VAL | GLU | LEU | LEU | LEU | ASP | GLN | ALA | ||||
| 10 | LEU | LEU | ALA | GLU | ARG | GLY | THR | LEU | GLU | ASP | ||||
| 11 | PRO | ASN | LEU | PHE | ILE | ARG | ARG | MET | ASN | GLN | ||||
| 12 | LEU | LEU | VAL | SER |
Samples:
sample_1: The CTD of E coli Hsp90, [15N, IMATLV-labeled], 600 uM; Phosphate 50 mM; NaCl 100 mM
sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - data analysis
NMRViewJ - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 600 MHz